Effects of pioglitazone on plaque stability and neovascularization in atherosclerotic plaque in rabbits
10.13699/j.cnki.1001-6821.2017.12.016
- VernacularTitle:吡格列酮对粥样动脉硬化模型的斑块稳定性及新生血管形成的作用
- Author:
Zhong-Shan LI
1
;
Lei SHI
;
Mei NI
Author Information
1. 东营胜利石油管理局胜利医院心内科
- Keywords:
pioglitazone;
plaque stability;
neovascularization;
atherosclerosis
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(12):1127-1130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of pioglitazone on plaque stability and neovascularization in atherosclerotic plaque.Methods New Zealand rabbits were selected and randomly divided into experimental group (n =20) and model group (n =20).The atherosclerosis model by feeding high fat diet for 6 weeks,ordinary feed,high fat diet for 6 weeks and 4 weeks of feeding method to build atherosclerosis model.The first week of the experimental group were fed to the in the high fat diet with pioglitazone 10 mg · kg-1,until the end of the experiment.In eighth weeks and eighteenth weeks,blood samples were taken.The hypersensitive C-reaction protein(CRP),blood glucose,blood lipid in two groups were determined by biochemical detection kit.The level of matrix meta-lloproteinase-9 (MMP-9) was detected by ELISA.Results The macrophage proportion in model group and the experimental group were (29.26 ± 3.94)％,(8.78 ± 4.01)％,the difference was statistically significant (P ＜ 0.05).The plaque area in model group and experimental group were (19.24 ± 10.57) × 10-2,(3.25 ±2.42) × 10-2 mm2,the difference was statistically significant (P ＜0.05).The number of neovascularization in model group and experiment group were 163.03 ± 72.96,79.86 ± 12.78,the difference was statistically significant (P ＜ 0.05).Animal 18 F-FDG uptake:average standard uptake (SUV mean) in model group and experimental group were 0.80 ± 0.08,0.64 ± 0.06,advanced SUVmean were 1.02 ± 0.60,0.50 ± 0.12,the differences were statistically significant (P ＜ 0.05).Metaphase SUVmax in model group and experimental group were 1.00 ± 0.07,0.84 ±0.06.Advanced SUVmax were 1.28 ±0.12,0.61 ±0.08,the differences were statistically significant (P ＜0.05).Conclusion Pioglitazone can reduce the degree of inflammation in the plaque,strengthen the plaque stability,reduce the production of new blood vessels and reduce the damage of vulnerable plaques,thus play the role of anti-atherosclerosis.
