Preparation and inhibitory effect of sirolimus sustained-release microsphere on lymphocyte proliferation
10.13699/j.cnki.1001-6821.2017.10.018
- VernacularTitle:西罗莫司缓释微球的制备及其对淋巴细胞增殖的抑制作用研究
- Author:
Wei LIU
1
;
Xiao-Tong WANG
;
Lei YANG
;
Jian-Hua WANG
Author Information
1. 首都医科大学附属北京世纪坛医院药剂科
- Keywords:
sirolimus;
sustained-release microsphere;
lymphocyte proliferation
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(10):933-935
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the preparation and inhibitory effect of Sirolimus sustained-release microsphere on lymphocyte proliferation.Methods The sirolimus sustained-release microspheres were prepared by the emulsion solvent evaporation.The morphology of microspheres were observed by transmission electron microscope.The inhibitory rate of sirolimus sustained-release microspheres on human lymphocyte were detected by MTT assay.Results The average particle size of the microspheres was (186.73 ± 12.62) μm,drug loading was (28.74 ± 2.63)％,encapsulation efficiency was (73.53 ± 7.12)％.Sirolimus sustained-release microspheres can stably release in vitro,the cumulative release rate of 24 h was 47.3％,the cumulative release rate of 168 h was 93.15％.After the culture of 12,24,48,72,96 h,the inhibition rate of proliferation of the microspheres was 18.28％,33.96％,50.24％,73.91％,83.02％.Conclusion Sirolimus sustained-release microspheres had good sustained-release effect in vitro and inhibition of lymphocyte proliferation,providing the basis for the new formulation of sirolimus.
