Function of Sinisan on hippocampal molecular expression in rats with post-traumatic stress disorder
10.13699/j.cnki.1001-6821.2017.10.015
- VernacularTitle:四逆散对创伤后应激障碍大鼠海马组织分子表达的作用
- Author:
Rui CAO
1
;
Jiang-Tao NIU
;
Yue-Feng LI
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Sinisan;
post-traumatic stress disorder;
hippocampus;
HPLC
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(10):921-924
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function mechanism of the Sinisanon on hippocampal molecular expression in rats with post-traumatic stress disorder.Methods Fifty SD rats were divided equally into 5 groups:blank control group,model group,negative control group group,positive control group and experimental group.The control blank group did not copy the model,do not receive treatment,the normal feeding.Model group,repetitive post-traumatic stress disorder model was induced by current stimulation,but not treated.Negative control group,equal volume of 0.9％ NaCl.Positive control group (paroxetine hydrochloride 0.42 mg· mL-1) and experimental group (Sinisan,Containing crude drug 0.24 g · mL-1).It given to drugs one hour before the model establishment.The rats were administered with 10 mL · kg-1,once a day,for a total of 7 d.At the end of 5 hours after the last administration,the hippocampus tissues were collected.Fingerprints were used to compare the difference peaks of hippocampus tissue in the rats with post-traumatic stress disorder.High performance liquid chromatography conditions:the gradient elution.The chromatograph condition:Agilent HC-C1s (4.6 mm × 250 mm,5 μm),speed of flow 0.8 mL · min-1;flowing A is methanol,flowing B is acetonitrile,flowing C is 0.05％ phosphorus acid water;wavelength of detection 260 nm;column warm 23 ℃.Results Compared with the blank control group,the model group is increased by 2nd,3rd,4th,6th and 7th chromatographic peak.Compared with the blank control group on peak area of 1 th (220.40 ± 9.25) mAU × min,5 th (86.27 ± 7.39) mAU × min,peak area in model group 1th (82.50 ± 9.60) mAU × min were significantly decreased (P ＜ 0.05),5th peak area (123.07 ± 9.28) mAU × min was significandy higher (P ＜ 0.05).Compared with model group,the peak area at the time of 1th (95.50 ±9.89) mAU ×min,5th (119.30 ±9.93) mAU ×min in negative controlgroup,it did not change significantly from chromatographic peak (P ＞ 0.05).Compared with negative control group,positive control group and experimental group disappear by 2nd,3rd,4th,6th and 7th peak,and 1th (230.42 ± 11.21),(234.80 ± 12.47) mAU ×min peak area was significantly higher (P ＜0.05) while 5th (71.29 ± 1.82),(72.16 ±2.55) mAU × min peak area were significantly decreased (P ＜ 0.05).Compared with positive control group,experimental group increased by 8th and 9th,it hint the two peaks may be chemical constituents or metabolites in the organism of Sinisan.Conclusion The Sinisan have significant positive effect on hippocampal molecular expression.
