Effect of knockdown microRNA-214 on drug resistance of different lymphoma cell types
10.13699/j.cnki.1001-6821.2017.10.011
- VernacularTitle:microRNA-214的下调表达对不同种类淋巴瘤细胞耐药性的影响
- Author:
Jian-Zhong CHEN
1
;
Xiu-Qin JIA
Author Information
1. 聊城市人民医院药学部
- Keywords:
lymphoma;
MicroRNA-214;
multidrug resistance
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(10):905-908
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the function of microRNA-214 on the drug resistance of different lymphoma cell types.Methods The Jurkat cells,OCI-Ly3 cells and Raji cells were selec-ted as the experimental groups.The healthy peripheral blood lymphocytes (Scr) were selected as the control group.The cells were miR-214 knockdown and stimulated with different drugs.The expression of miR-214 in different types of lymphoma ceils was detected by real-time PCR.The cell viability of lymphoma cells after knockdown miR-214 was detected by CCK-8.The drug sensitivity was detected by methyl thiazolyl tetrazolium colorimetric method(MTT) assay.The BCL11B protein levels was detected by Western blot.The luciferase reporter assay was used to detect whether BCL11B was the target gene of miR-214.Results The miR-214 expression in the experimental groups of Jurkat,Oci-ly3 and Raji cells were 27.21 ± 1.37,17.13 ±2.11,19.31± 2.46,which were significantly higher than those in control group of 1.17 ± 0.28,the difference was statistical significance (P ＜ 0.05).After knockout miR-214,compared with the cell viability in control group (86 ± 12)％,the cell viability of Jurkat,OCI-Ly3 and Raji were (42 ± 13)％,(65 ± 11)％,(58 ± 7)％,the difference were significantly decreased (P ＜ 0.05).The drug sensitivity of Scr cells (control group) to doxorubicin,cyclophosphamide,daunorubicin,mito-mycin C and vincristine were 0.46 ± 0.11,1.96 ± 0.13,1.15 ± 0.22,3.90 ± 0.52,0.52 ± 0.15,respectively.The drug sensitivity of Jurkat cells to five drugs were 0.32 ±0.07,0.76 ±0.16,0.73-±0.13,2.36 ±0.16,0.37 ±0.06.The drug sensitivity of OCI-Ly3 cells to the five drugs were 0.28 ±0.06,0.91 ±0.12,0.55 ±0.09,2.01 ±0.36,0.23 ± 0.07,respectively.The drug sensitivity of Raji cells to five drugs were 0.36 ± 0.04,0.82 ± 0.08,0.64 ± 0.07,3.31 ±0.21,0.35 ±0.05,respectively.Which indicated that the drug sensitivity of lymphoma cells to various drugs was significantly decreased after the inhibition of miR-214 expression.The BCL11B was the direct target of miR-214.Conclusion Inhibition of miR-214 expression could decrease the cell viability of lymphoma cells and decrease the multidrug resistance of leukemia lymphocytes,which may be related to the miR-214 knockdown on the expression of BCL11 B.
