Regulating action of unsaturated fatty acids on human gastric cancer cells protein kinase B signaling pathways
10.13699/j.cnki.1001-6821.2017.10.009
- VernacularTitle:不饱和脂肪酸对人胃癌细胞蛋白激酶B信号通路的调控作用
- Author:
Fa-Rong YU
1
;
Wang-Jun CHEN
;
Zhong-Jian LEI
;
Xiu-Zhen LIAN
;
Ming-Ren XIE
;
Deng-Lou LI
;
Bo YANG
;
Yun-Xuan GUO
Author Information
1. 甘肃政法学院甘肃省证据科学技术研究与应用重点实验室
- Keywords:
unsaturated fatty acids;
human gastric cancer cell;
severe combined immunodeficiency mouse
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(10):897-900
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulating action of unsaturated fatty acids on human gastric cancer cells protein kinase B (PKB/Akt)signaling pathways.Methods The gastric cancer tissue of adult patients with gastric cancer was isolated and transplantation in severe combined immunodeficiency (SCID) mice.Separation cells from gastric cancer transplanted tumor by digestive enzyme were cultured.They were divided into five groups:three doses experimental groups (unsaturated fatty acid 0.3,0.6,1.2 mg · L-1),control group (5-fluorouracil 10 mg · L-1),solvent control group (dimethyl sulfoxide 30 μL · L-1),cultivate 48 h after the treatment.The toxic effects of the unsaturated fatty acid on human gastric cancer cells were detected by 3-(4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2-H-tetrazolium bromide (MTT) method.The levels of p21,murine double minute2 (MDM2),PKB/Akt in tumor cells were detected by the method of enzyme linked immunosorbent assay.Results Compared to the control group,the human gastric cancer cells proliferation inhibition rate in high,moderate,low,doses experimental groups were increased by 31.7％,55.51％,68.28％,respectively.The levels of p21 and MDM2 in that three groups were increased by 4.39％,80.12％,161.36％ and 19.81％,97.76％,207.35％,respectively.The level of PKB/Akt in that three groups was reduced by 13.31％,57.69％,78.71％,respectively.Conclusion The unsaturated fatty acids on human gastric cancer cells proliferation inhibition mainly related to elevated p21,MDM2 levels within the tumor cells,inhibit the activity of PKB/Akt signal pathway.
