Determining the concentration of cytosine arabinoside and its metabolites 1-β-D-arabinofuranosyluracil in human blood by HPLC
10.13699/j.cnki.1001-6821.2017.09.016
- VernacularTitle:高效液相色谱法同时测定人全血中阿糖胞苷及其代谢物阿糖尿苷的浓度
- Author:
Juan GAO
1
;
Jie WANG
;
Qi-Zhou ZHANG
;
Xue-Ting MAI
;
Liang TENG
Author Information
1. 新疆医科大学药学院
- Keywords:
high performance liquid chromatography;
cytosinearabinoside;
1-β-D-arabinofuranosyluracil;
blood drug concentration
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(9):828-831
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for simultaneous determining the concentration of cytosine arabinoside (Ara-C) and 1-β-D-arabinofuranosyluracil (Ara-U) in human blood by HPLC-UV.Methods Both Ara-C and Ara-U were extracted from blood by protein deposition method and determined by HPLC,and separated on a Cosmosil Cholester(4.6 mm ×250 mm,5 μm) with mobile phase consisted of methanol∶ (0.01 mol · L-1,pH =6.7) phosphate buffer solution (3∶ 97) at a flow rate of 1 mL · min-1.The detective wavelength was 280 nm and the column temperature was maintained at 25 ℃.Results]he linear range of Ara-C was 1.55-198.00 μg · mL-1 (r =0.9985) and Ara-U was 1.34-171.00 μg · mL-1 (r =0.997 8) with the formulation y =16417.59x-14511.01 and y =4468.72x-1521.15 respectively.When S/N≥3,the detection limit of Ara-C and Ara-U were 0.79,0.67 μg · mL-1.The recovery rate of Ara-C and Ara-U was respectively 74.84％-87.74％ and 77.76％-88.92％,with both intra-day RSD less than 15％ and about inter-day,the high and medium concentration RSD both were less than 15％ and 20％.Conclusion The established method was accurate and specific,meanwhile,it has high recovery,litde interference and low cost,thus it's applicable for the clinical determination of blood concentration of Ara-C and Ara-U.
