HPLC-MS/MS method for the determination of sufentanil in human plasma
10.13699/j.cnki.1001-6821.2017.09.015
- VernacularTitle:液质联用法检测人血浆中舒芬太尼浓度
- Author:
Wei LIU
1
;
Xin XIONG
;
Xian-Hua ZHANG
;
Yan ZHAO
;
Qiong WU
;
Suo-Di ZHAI
Author Information
1. 北京大学第三医院药剂科
- Keywords:
sufentanil;
fentanyl;
HPLC-MS/MS;
plasma
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(9):825-827,831
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a sensitive,rapid and robust high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method for the analysis of sufentanil in human plasma.Methods Sufentanil in human plasma was extracted by tert-butyl methyl ether and analyzed by HPLC-MS/MS with fentanyl as the internal standard (IS).Multiple reactions monitoring was used,transitions and collision energies were as follows:sufentanil m/z 387.2→m/z 238.2(27 V),fentanyl m/z 337.2→m/z 158.2 (37 V) in positive electrospray ionization mode.The chromatographic separation was performed on Waters Atlantis Hilic Silica column (2.1 mm ×-50 mm,5 μm),with a flow rate at 0.3 mL · min-1.A gradient elution was applied,mobile phase was 10 mmol · L-1 ammonium formate grid acetonitrile,both containing 0.1％ formic acid.Results The calibration curve was y =9.28 × 10-4x + 1.86 × 10-3 (r =0.997 4),over the concentration range of 2-500 pg · mL-1 was linear,and the lower limit of qualification (LLOQ) was 2 pg · mL-1.The intra-day and inter-day precisions were all less than 10％.Conclusion The assay was successfully applied to the analysis of sufentanil in patients which can provide reliable results.
