HPLC method for the determination of ciprofloxacin concentration in rat plasma and bronchoalveolar lavage fluid
10.13699/j.cnki.1001-6821.2017.09.013
- VernacularTitle:高效液相色谱法测定大鼠血浆和肺泡灌洗液中环丙沙星浓度
- Author:
He-Kun MEI
1
;
Jin WANG
;
Dong CHAI
;
Rui WANG
;
Lei XIA
;
Wen-Tao NI
Author Information
1. 解放军总医院药物临床研究中心
- Keywords:
HPLC;
ciprofloxacin;
plasma concentration;
bronchoalveolar lavage fluid
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(9):817-820
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a sensitive and reliable HPLC method for the determination of ciprofloxacin in rat plasma and bmnchoalveolar lavage fluid (BALF),and to apply this method for Pharmacokinetics.Methods Plasma sample and BALF sample were precipitated by methanol and dilution with water respectively,moxifloxacin was used as internal standard,the analytes were separated on an Agilent SB-C18 column (4.6 mrn × 150 mm,5 μm)using the mobile phase of acetonitrile and 0.1％ formic acid in water at a flow rate of 1.0 mL · min-1,column temperature was 25 ℃.Results Chromatograms showed no endogenous interfering peaks in the respective blank human plasma and BALF samples.The calibration curves for plasma and BALF were linear over a wide concentration range of 1.00-100.00 μg · mL-1 (r =0.999 6)and 0.05-2.00 μg · mL-1 (r =0.998 5) with a lower limit of quantification of 1 μg · mL-1 and 0.05 μg · mL-1.The Inter-day and intra-day RSD of plasma and BALF were 0.49％-3.77％,2.21％-5.00％,7.72％-8.83％,6.84％-9.51％,respectively.The extraction recovery of plasma and BALF were 104.61％-113.29％ and 100.65％-103.96％.Data of stability study fitted the request.Conclusion The method is simple,sensitive and accurate for the determination of ciprofloxacin in human plasma and BALF samples.It could be widely used for pharmacokinetic study of ciprofloxacin.
