Protective effects of Salvianolic acid on myocardial ischemic injury of rats from the aspect of inhibiting inflammatory reaction
10.13699/j.cnki.1001-6821.2017.09.007
- VernacularTitle:丹参多酚酸对心肌缺血大鼠血清及心脏组织炎症因子的保护作用
- Author:
Xiu-Ping QIN
1
;
Meng-Xi XU
;
Rong-Rong HAO
;
Mi-Xia ZHANG
;
Peng-Wei ZHUANG
;
Guang-Zhi CUI
;
Yan-Jun ZHANG
Author Information
1. 天津中医药大学天津市中药药理重点实验室
- Keywords:
Salvianolic acid;
myocardial ischemia;
inflammatory cytokine;
myocardial preservation
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(9):794-797
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of Salvianolic acid on myocardial ischemic injury of rats from the aspect of inhibiting inflammatory reaction.Methods Sixty Sprague Dawley (SD) male rats were randomly divided into four groups (n =15per group):sham operation group,model group,experimental-high (36 mg · kg-1) group,experimental-low (18mg · kg-1) group with intraperitoneal injection dosing.Same amount of distilled water was injected to sham operation group and model group,lasting for 4 days.Myocardial ischemia model was established by coronary artery ligation of left anterior descending (LAD) branch after the last administration.Six hours after the operation,the blood of eye canthus was collected to assay the levels of creatine kinase(CK),MB isoenzyme of creatine kinase (CK-MB) and lactate dehydrogenase (LDH) in serum.The heart was collected 24 h after the operation,stained by hematoxylin eosin (HE)and checked the pathological change of the cardiac muscle tissue,assayed the cardiac troponin (cTnT),tumor necrosis factor α (TNF-α) in serum and myeloperoxidase (MPO) was measured in myocardial tissue homogenate.Results After 6 hours' myocardial ischemia,the serum CK in sham operation group was (529.61 ± 141.93) U · L-1,CK-MB was (708.12 ±385.93) U · L-1,LDH was (330.12 ±158.38) U · L-1 and cTnT was (294.12 ±55.10)pg· mL-1 after 24 hours' myocardial ischemia.The serum CK in model group was (996.42 ± 413.42) U · L-1,CK-MB was (1346.11 ±558.30) U · L-1,LDH was (520.12 ±154.76) U · L-1and cTnT was (513.00 ±69.71)pg · mL-1after 24 hours' myocardial ischemia.The serum CK in experimental-low and experimental-high groups were (499.45 ±159.33),(514.91 ±98.82)U · L-1,CK-MB were (831.42 ±385.11),(592.10 ±206.32)U·L-1,LDH were (462.62 ±229.68),(437.72 ± 175.80)U· L-1,and cTnT were (431.12 ±106.00),(338.80 ± 76.92)pg · mL-1after 24 hours' myocardial ischemia.Compared with the sham operation group,the CK,CK-MB and LDH value in the serum of model group increased significantly (P ＜ 0.05).Compared with the model group,the salvianolic acid significantly both can decrease the CK and CK-MB in serum (P ＜ 0.05).Compared with sham operation group,the cTnT value in the serum of model group increased significantly (P ＜ 0.05).Compared with model group,high dosage of salvianolic acid could significantly decrease the cTnT value (P ＜ 0.05).After 24 hours'myocardial ischemia,the TNF-α in serum of sham operation group was (118.90 ± 17.58) pg · mL-1,the MPO in heart tissue was (32.25 ± 3.75) U · L-1.The TNF-α in serum of model group was (156.00 ± 28.24) pg · mL-1,the MPO in heart tissue was (104.83 ±22.87) U · L-1.The TNF-α in serum of experimental-low and experimental-high groups were (136.05 ± 34.00),(125.06 ± 30.13) pg · mL-1,the MPO in heart tissue were (91.70 ±21.57),(68.00 ± 18.47) U · L-1.Compared with sham operation group,the TNF-α values in the serum and the MPO value in model group increased significantly (P ＜ 0.05).Compared with model group,these values of experimental-high group decreased with significantly (all P ＜ 0.05).Conclusion Salvianolic acid showed the protection of myocardial tissue,which also could significantly reduce the myocardial cell injury and inflammatory cell infiltration of cardiac myocytes,which could be related with the decreased levels of TNF-α and MPO.
