Comparison the effect of clearing-heat and drying-damp on root and stem leaves of Scutellaria baicalensis from different areas in Gansu province
10.13699/j.cnki.1001-6821.2017.08.016
- VernacularTitle:甘肃不同产地黄芩的根和茎叶清热燥湿作用比较
- Author:
Xiu-Juan YANG
1
;
Zhi-Jun YANG
;
Jin-Bao ZHANG
;
Tao DU
;
Kai CHEN
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Scutellaria baicalensis;
clear heat and dry damp;
interleukin-4;
interferon-γ;
motilin;
gastrin
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(8):730-733,744
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the difference effects on clearing-heat and drying-damp of root and stem leaves of Scutellaria baicalensis from different areas in Gansu province.Methods One hundred SD rats were randomly divided into ten groups,including blank group,model group,Scutellaria baicalensis from Longxi Ⅰ and Ⅱ groups,Weiyuan Ⅰ and Ⅱ groups,Zhangxian Ⅰ and Ⅱ groups,Minxian Ⅰ and Ⅱ groups,Ⅰ group means root and Ⅱ group means stem leaves,each group had ten rats.The blank group rats were given ordinary feed and saline(10 mL · kg-1) in the natural environment.Rats in other groups were given fat feed and white wine [Red Star Zhenpin Erguotou (56 °),5 mL · kg-1] once daily,and placed into manual climatic box for 21 days.On the 22th day,the coli (1 × 109/mL) were given once (10 μL · g-1),and enhancement of infection after 4 hours (5 μL · g-1).The animal models were given the roots and steams leaves of Scutellaria baicalensis decoction (10 g · kg-1) by once daily with 7 days.The rats were placed into natural environment and given ordinary feed.The syndrome performance of rats in each group were observed.The contents of serum and stomach-intestine interleukin-4 (IL-4),interferon-γ (IFN-γ),motilin(MTL),gastrin(GAS)in rats were detected by ELISA.Results With the treatment of the roots and steams leaves of Scutellaria baicalensis decoction,the serum contents of IL-4 and IFN-γ were (25.36--2.56),(703.53-± 29.36);(31.54.± 3.81),(810.90 ± 24.07);(26.88 ± 2.91),(679.02±30.72);(30.81 ±3.19),(802.44 ±20.69);(26.61 ±3.94),(702.90 ±14.86);(32.45 ±5.12),(815.36 ± 34.78);(27.06 ± 4.01),(685.66 ± 42.05);(31.76 ± 3.98),(808.95 ± 38.22) ng· L-1 respectively in each drugs group,which were obviously lower than that of model group with (38.09 ± 1.41),(1203.90 ± 32.24) ng· L-1 on contents of IL-4 and IFN-γ.The contents of GAS and MTL in serum were (34.14 ± 1.47),(475.82 ±7.80);(35.12 ±3.40),(436.25±18.24);(34.77 ±2.65),(475.04 ±28.79);(33.28 ±3.58),(447.81±8.26);(35.26 ±4.89),(473.09 ±28.64);(36.02 ±5.12),(440.52 ±23.55);(35.20 ±4.55),(478.58 ± 31.24);(34.88 ± 5.07),(438.08 ± 25.43)ng · L-1 respectively in each drugs group,which were obviously higher than that of model group with (28.61 ± 0.12),(404.16 ± 18.48)ng · L-1 on contents of GAS and MTL,all about with statistical difference (all P ＜0.05).Conclusion There was a significant effect on clearing-heat and drying-damp of root and stem leaves of Scutellaria baicalensis from different areas in Gansu province.
