Effects of dl-3-n-butylphthalide on expression of Caspase-3 and Caspase-9 mRNA and protein in rats with acute severe carbon monoxide poisoning
10.13699/j.cnki.1001-6821.2017.08.014
- VernacularTitle:丁苯酞对大鼠急性一氧化碳中毒后半胱氨酸蛋白酶-3和半胱氨酸蛋白酶-9表达的影响
- Author:
Zhun ZHOU
1
;
Guo-Qian LI
;
Jie-Hua WANG
;
Xiao-Xia YANG
;
Zhu-Quan HONG
Author Information
1. 福建医科大学附属泉州第一医院神经内科
- Keywords:
dl-3-n-butylphthalide capsule;
carbon monoxide poisoning;
Caspase-3;
Caspase-9
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(8):722-725
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of dl-3-n-butylphthalide(NBP) on the expression of Caspase-3 and Caspase-9 in the rat brain tissue after acute severe carbon monoxide poisoning.Methods Male Sprague-Dawley rats were randomly divided into three groups:normal group,model group and experimental group.The acute severe carbon monoxide poisoning model was made by the literature.Rats in experimental group were given 80 mg · kg-1 NBP,and rats in normal group and model group were given same volume of saline by gavage one time a day for seven days.Immunohistochemistry were used to observe the positive cells changes of Caspase-3 and Caspase-9 after CO poisoning among the three groups.RT-PCR and Western blotting were used to detect the expression levels of Caspase-3 and Caspase-9's mRNA and protein.Results In normal group,the expression of Caspase-3 and Caspase-9 positive cells were 4.98-± 1.34,8.65 ± 1.89,Caspase-3 and Caspase-9 of mRNA were 0.19 ± 0.02,0.18 ± 0.03,Caspase-3 and Caspase-9 of proteins were 0.09-± 0.01,0.08 ± 0.0i.In model group,Caspase-3 and Caspase-9 positive cells were 29.43 ± 4.53,40.45 ± 5.23,Caspase-3 and Caspase-9 of mRNA were 0.47 ±0.05,0.75 ± 0.05,Caspase-3 and Caspase-9 of proteins were 0.78 ± 0.05,0.86 ± 0.07.Compared with normal group,the expressions of Caspase-3 and Caspase-9 were increased significantly in the model group (P ＜ 0.05).In experimental group,Caspase-3 and Caspase-9 positive cells were 22.26 ± 3.12,25.09 ±4.25,Caspase-3 and Caspase-9 of mRNA were 0.35 ±0.05,0.41 ±0.05,Caspase-3 and Caspase-9 of proteins were 0.37 ± 0.02,0.45 ± 0.02.Compared with model group,the Caspase-3 and Caspase-9 expressions were dowm regulated in rats of experimental group (P ＜ 0.05).Conclusion NBP seemed to play an important role in alleviation of brain damage via decreasing the expression of Caspase-3 and Caspase-9 and inhibiting the apotosis of cells.
