Effect of rosuvastatin on expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 following focal cerebral ischemia-reperfusion
10.13699/j.cnki.1001-6821.2017.08.010
- VernacularTitle:瑞舒伐他汀对大鼠脑缺血再灌注后基质金属蛋白酶9和组织金属蛋白酶抑制物1表达的影响
- Author:
Jie-Hua WANG
1
;
Guo-Qian LI
;
Ying PAN
;
Xiu-Xiu XU
;
Shu-Zeng CHEN
Author Information
1. 福建医科大学附属泉州第一医院神经内科
- Keywords:
rosuvastatin;
cerebral ischemia-reperfusion;
matrix metalloproteinase-9;
tissue inhibitor of metalloproteinase-1
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(8):706-709
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of rosuvastatin on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the brain tissue of cerebral ischemia-reperfusion rats and discuss its cerebral protective role.Methods Rats were randomly assigned into three groups:sham operation group(sham group),model group and test group.The rats in the test group were lavaged with 5 mg · kg-1 · d-1 rosuvastatin for 10 consecutive days before the model setting-up.The rats in the sham group and model group were fed normally.The model rats with middle cerebral artery occlusion were prepared by the modified Zea-Longa suture method.After vascular occlusion 2 h,remove the suture to form reperfusion.The rats in sham group received similar surgical treatment,but didn't insert the suture.The levels of MMP-9 and TIMP-1 were measured by RT-PCR,immunohistochemistry and Western blotting.Results The expression of MMP-9 mRNA in model group and test group were (0.45 ± 0.04) and (0.31-± 0.04) respectively,and the expression of TIMP-1 mRNA in model group and test group were (0.39 ± 0.05) and (0.52 ± 0.06) respectively,the difference were statistically significant (P ＜ 0.05).The positive cell number and protein expression of MMP-9 and TIMP-1 in model group and test group were also statistically significant (P ＜ 0.05).Conclusion Rosuvastatin can protect the brain injury through adjusting the expression of MMP-9 and TIMP-1.
