Effect of Ginkgo biloba flavone on apoptosis factor in model mice with acute cerebral infarction
10.13699/j.cnki.1001-6821.2017.08.009
- VernacularTitle:银杏叶黄酮对急性脑梗死模型小鼠凋亡因子的影响
- Author:
Wei XIANG
1
;
Su-Yue PAN
;
Xiao-Na WU
;
Gao-Quan LUO
;
Bing-Mei DENG
Author Information
1. 南方医科大学附属南方医院神经内科
- Keywords:
Ginkgo biloba flavone;
acute cerebral infarction;
hypoxia inducible factor-1;
tumor necrosis factor-α
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(8):703-705
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Ginkgo biloba flavone on the expression of hypoxia inducible factor-1 α (HIF-1 α),tumor necrosis factor-α (TNF-α) and Caspase-3 in serum of model mice with acute cerebral infarction.Methods Twenty-four female Kunming mice were selected,8 rats were randomly selected as sham operation group,and the remaining mice were established acute cerebral infarction model.Sixteen mice were successfully established the disease,and randomly divided into model group and experimental group,each group contained 8 mice.In experimental group,mice were treated by intraperitoneal injection of Ginkgo biloba flavone (20 mg · kg-1) once a day;mice in sham operation and model groups received intraperitoneal injection of physiological saline once a day,for 7 days.After experiment,all the mice were killed and extract the serum for detecting the expression level of HIF-1α,TNF-α and Caspase-3 by ELISA assay.Results The TNF-α levels of serum in the sham operation group,model group and experimental group were (5.16 ± 1.04),(33.02 ± 20.34),(22.64 ± 10.17)ng · mL-1 respectively.The serum HIF-1α levels of serum in above three groups were (508.94 ± 123.18),(735.19 ± 324.05),(626.24 ±212.76) ng· mL-1 respectively.The serum Caspase-3 levels of serum in above three groups were (6.54 ±0.24),(12.23 ± 3.45),(10.24 ± 1.58)ng · mL-1 respectively.The TNF-α,HIF-1 α and Caspase-3 expression levels of the sham operation group was significantly higher than that of model group,the difference was statistically significant (P ＜ 0.05).The TNF-α,HIF-1 α and Caspase-3 expression levels of the experimental group was significantly lower than that of model group,the difference was statistically significant (P ＜ 0.05).Conclusion Ginkgo biloba flavonoids could obviously down-regulate the expression level of HIF-1 α,TNF-α and Caspase-3.
