Determination of arbidol concentration in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2017.07.016
- VernacularTitle:高效液相色谱-质谱联用法测定人血浆中阿比朵尔的浓度
- Author:
Ying-Fu ZHANG
1
;
Guang-Tao HAO
;
Long-Mei CHENG
;
Guo-Fang BI
;
Li-Jun ZHANG
;
Yuan-Yuan ZHANG
;
Ze-Yuan LIU
;
Heng-Yan QU
Author Information
1. 军事医学科学院附属医院临床药理学研究室
- Keywords:
arbidol;
HPLC-MS/MS;
plasma concentration
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(7):631-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the arbidol concentration in human plasma by established HPLC-MS/MS method.Methods The plasma was treated with protein precipitation,arbidol-d6 was used as internal standard,chromatographic column was Agilent Eclipse XDB-C18 column (2.1 mm × 50 mm,5 μm),column temperature was 30 ℃,mobile phase was methanol-0.1％ formic acid,isocratic elution,flow rate was 0.25 mL · min-1,mass concentration of the sample was measured with positive ion scan and multiple reaction monitoring mode.The specificity,lower limit of quantitation and standard curve,precision and recovery rate and stability as well as the matrix effect were investigated.Results The standard curve of arbidol was y =1.52 × 10-1x-1.38 × 10-3 (r =0.999 7),and in 10-1000 ng· mL-1 good linear relationship,the lowest concentration was 10 ng · mL-1.Intra-day and inter-day RSD of blood samples were less than 15％,the average recovery was ＞95％,and stability was good.Conclusion The method is simple,rapid,sensitive and accurate,specific,suitable for the determination of arbidol concentration in human plasma.
