Determination of pregabalin in epileptic patients by liquid chromatography tandem mass spectrometry
10.13699/j.cnki.1001-6821.2017.07.015
- VernacularTitle:HPLC-MS/MS法测定癫痫患者体内普瑞巴林的血药浓度
- Author:
Yan-Yan LIU
1
;
Chang-Chen WAN
;
Chun-Hua ZHOU
;
De-Qiang LI
;
De-Zhi KONG
Author Information
1. 河北医科大学药学院药物分析教研室
- Keywords:
pregabalin;
HPLC-MS;
plasma;
epileptic patient
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(7):628-630,637
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC-MS/MS method for the determination of Pregabalin in epileptic patients.Methods Analysis was performed on a Diamonsil C18column (4.6 mm × 150 mm,5 μm)eluted with methanol and water (0.05％ formic acid) in a gradient program.The flow rate was 1 mL · min-1,the injection volume was 20 μL,and the column temperature was 30 ℃.The multiple-reaction monitoring scanning (MRM) was employed for the quantification with switching electrospray ion source polarity in negative mode.The ion spray voltage was set at 5500 V and the turbo spray temperature was maintained at 650 ℃.The specificity,calibration curve,limit of detection,precision,recovery and stability of the method were investigated.20 patients with epilepsy were selected,and the serum concentration of pregabalin was determined by HPLC-MS/MS after oral administration of 75 mg pregabalin for one hour.Results Pregabalin showed a good linearity in the ranges of 13.76-5270.00 ng · mL-1,the lower limit of quantification was 0.65 ng · mL-1.The average recoveries varied from 94.7％-106.2％.The inter and intra-day RSD were less than 15％.Pregabalin was proved to be stable under study station.The concentration of pregabalin in epileptic patients ranged from 1.76 to 2.78 μg · mL-1.Conclusion The method is simple,rapid,and sensitive,which could be used for the determination of pregabalin in epileptic patients.
