Expression and clinical significance of calcium-binding protein Calgranulin A and Calgranulin B in non-small cell lung cancer
10.13699/j.cnki.1001-6821.2017.07.012
- VernacularTitle:钙结合蛋白Calgranulin A和Calgranulin B在非小细胞肺癌中的表达及临床意义
- Author:
Wei MA
1
;
Chao-Nan MA
;
Nan-Nan ZHOU
;
Yi-Jie ZHANG
Author Information
1. 河南大学淮河医院呼吸内科
- Keywords:
Calgranulin A protein;
Calgranulin B protein;
lung squamous cell carcinoma;
lung adenocarcinoma
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(7):616-619
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression characteristics of calcium-binding protein S100A8 (Calgranulin A) and S100A9 (Calgranulin B) in lung squamous cell carcinoma and lung adenocarcinoma and their correlations with clinical data.Methods Twenty paired snap frozen cancer tissue and para-cancer tissue of lung cancer patients were collected form Jan 2013 to Dec 2014 in our hospital.And other 100 cancer paraffin wax specimen,20 para-cancer paraffin wax specimen were retrospectively collected from Jan 2007 to Dec 2009.The expressions of S100A8 and S100A9 were analyzed by PCR,Western blot and immunohistochemistry assay.The association between S100A8,S100A9 expression and patients clinical characteristics were analyzed.Results Positive rates of S100A8,S100A9 mRNA were 100.0％ for cancer tissue,significant higher than those of para-cancer tissue of 40.0％ and 85.0％ (P ＜0.05).Positive rates of S100A8,S100A9 protein were 100.0％ for cancer tissue,significant higher than those of para-cancer tissue of 20.0％ and 90.0％ (P ＜ 0.05).S100A8 positive expression rate was 28.6％ and 89.5％ for well differentiation and poor differentiation groups with statistical difference (P ＜ 0.05).The expression rate of S100A9 for stage Ⅰ positive was 73.0％ and the 100.0％ of S100A9 for stage Ⅲ/Ⅳ groups with statistical difference (P ＜ 0.05).Conclusion The expression levels of S100A8 and S100A9 in lung adenocarcinoma and lung squamous cell carcinona was higher than those in normal tissue,which were negatively correlated with the degree of differentiation of lung adenocarcinoma,and without correlation to the degree of differentiation of lung squamous cell carcinoma.The expression level of S100A9 was negatively correlated with tumor TNM stage.
