Antithrombotic effect of Ligustrazine injection combined with aspirin and clopidogrel in rabbits
10.13699/j.cnki.1001-6821.2017.07.011
- VernacularTitle:川芎嗪注射液与阿司匹林和氯吡格雷联用抗家兔血栓形成的作用
- Author:
Jin HE
1
;
Yun-Peng GONG
;
Bo-Long WANG
;
Chun-Lin CHEN
Author Information
1. 宜春学院化学与生物工程学院
- Keywords:
Ligustrazine;
thrombus formation in vitro;
platelet aggregation;
platelet activating factor;
cluster of differentiation 62 platelet
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(7):612-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti thrombotic effect of Ligustrazine injection combined with aspirin,clopidogrel in rabbits.Methods Forty-two healthy Japanese white rabbits were randomly divided into 7 groups:blank group,control A group,control B group,control-low dose herb group,control-high dose herb group,combined A group,combined B group.Blank group was given equal volume normal saline,control A group was given aspirin 5.13 mg· kg-1,control B group was given clopidogrel 3.85 mg · kg-1,control-low,high doses herb groups were given Ligustrazine injection 6.54,26.16 mg · kg-1,combined A group was given Ligustrazine injection 26.16 mg· kg-1 and aspirin 5.13 mg · kg-1,combined B group was given Ligustrazine injection 26.16 mg · kg-1 and clopidogrel 3.85 mg · kg-1.Ligustrazine injection was given for ten days through ear marginal vein and aspirin,clopidogrel were given for seven days through intragastric administration.On the last day of administration,heart blood was taken at 1 h after administration.Thrombus length and wet weight were measured by extracorporeal thrombus forming apparatus.The effect on the platelet aggregation rate induced by platelet activating factors (PAF) was measured by platelet aggregation instrument.And the content of platelet activating factors (PAF),cluster of differentiation 62 platelet (CD62p) and platelet factor-4 (PF-4) were detected by enzyme-linked immunosorbent assay.Results The throrobus length in combined B group was (7.4 ± 4.6) cm,which significantly reduced compared with that of blank group (22.8 ±2.3) cm (P ＜0.01).The wet weights of thrombus of control A,B group,control-high dose herb group and combined A,B group were (0.31 ± 0.04),(0.30 ± 0.05),(0.35 ±0.14),(0.28 ±0.08),(0.16 ±0.07) g,compared with that of blank group (0.55 ±0.10) g with significantly reduced(P ＜ 0.01).The platelet aggregation rates of control-low,-high doses herb groups and combined A,B groups induced by PAF were (43.3 ± 4.7) ％,(37.2 ± 4.7) ％,(35.5 ± 4.1) ％,(35.0 ± 3.2) ％,when compared with that of blank group (52.9 ± 4.4) ％,they reduced significantly (P ＜ 0.01).The platelet aggregation rates of combined A,B group were significantly lower than that of control-low dose herb group (P ＜ 0.05).The PAF contents of control-low,-high doses herb groups and combined A,B groups were (1043 ± 162),(1010 ± 189),(973 ± 159),(959 ± 178) ng · L-1,significantly lower than that of blank group (1510 ± 319) ng · L-1 (P ＜ 0.01).The CD62P contents of control A,B groups,control-low,-high doses herb groups and combined A,B groups were (156 ± 13),(130 ±21),(101 ±17),(85 ±8),(107 ± 18),(107 ± 19) ng · mL-1,significantly lower than that of blank group (234 ±69) ng · mL-1(P ＜ 0.01).The PF-4 content of control A,B groups,control-high dose herb group and combined A,B groups were (148 ± 14),(255 ± 14),(322 ±69),(242 ±26),(266 ±36) ng· mL-1,which significantly reduced compared with that of blank group (478 ±91) ng · mL-1 (P ＜0.05,P ＜0.01).Conclusion Ligustrazine injection combined with aspirin and clopidogrel can play a synergistic anti-thrombosis effect.
