Effect of Xingnaojing injection on myocardial enzyme and mitochondrial respiratory chain complex in rats with sepsis
10.13699/j.cnki.1001-6821.2017.07.010
- VernacularTitle:醒脑静注射液对脓毒症大鼠心肌酶和线粒体呼吸链复合物的作用
- Author:
Jin-Hua WANG
1
;
Di CHE
;
Dong CHEN
;
Qi-Yi ZENG
Author Information
1. 南方医科大学附属珠江医院儿科 广州510282
- Keywords:
Xingnaojing injection;
sepsis;
myocardial enzyme;
mitochondrial respiratory chain complex
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(7):609-611,623
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Xingnaojing injection on myocardial enzyme and mitochondrial respiratory chain complex in sepsis rats.Methods Seventy SD rats were randomly divided into 3 groups,10 for normal group,intraperitoneal injection of the same amount of normal saline as lipopolysaccharides (LPS).The other 60 SD rats were injected intraperitoneally with 10 mg· kg-1 LPS,prepared with sterile injection water and the concentration of LPS was 1 mg · mL-1 to establish sepsis model.The 30 rats were randomly selected as model group,while another 30 rats as experimental group were injected Xingnaojing.Both model group and experimental group were randomly selected 10 rats to observe in 6 h,24 h,48 h,respectively.The levels of myocardial enzyme and respiratory chain complex (complex Ⅰ),Ⅱ,Ⅲ,Ⅳ at different times in sepsis rats were observed.Results At 24 h,in experimental and model group,the level of creatine kinase (CK) were (1333.20-± 140.87),(1813.50 ± 243.72) U · L-1,the creatine kinase isoenzyme (CK-MK) in the two groups were (748.15 ± 119.02),(1167.92 ± 145.17)U · L-1,respectively.The levels of CK and CK-MK in the experimental group were significantly lower than those in model group(P ＜0.01).At 24 h,in experimental and model groups,the activity of myocardial mitochondrial complex Ⅰ in the two groups were (2.83 ± 0.15) and (1.84 ± 0.18) u · mg-1,complex Ⅲ in the two groups were(0.91 ±0.13) and(0.68 ±0.16) u · mg-1,complex 1W in the two groups were (19.43 ± 1.37) and(15.20 ± 1.79) u · mg-1,respectively.The levels of myocardial mitochondrial complex Ⅰ,Ⅲ and Ⅳ in the experimental group were significantly higher than those in model group (P ＜ 0.01) Conclusion Xingnaojing injection could significantly reduce the level of myocardial enzyme and improve the levels of complex Ⅰ,Ⅲ,Ⅳ.
