Effect of octadecenoic acid on human gastric cancer tissue with targeted role
10.13699/j.cnki.1001-6821.2017.06.016
- VernacularTitle:十八烯酸对人胃癌组织的靶向作用
- Author:
Fa-Rong YU
1
;
Bo YANG
;
Xiu-Zhen LIAN
;
Ming-Ren XIE
;
Deng-Lou LI
;
Shi-Shuang ZHANG
;
Yun-Xuan GUO
;
Wang-Jun CHEN
Author Information
1. 甘肃政法学院甘肃省证据科学技术研究与应用重点实验室
- Keywords:
octadecenoic acid;
human gastric cancer tissue;
severe combined immunodeficiency mouse
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(6):539-541,564
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the growth inhibition mechanism of octadecenoic acid on human gastric carcinoma transplanted tumor.Methods The cancer tissue was isolated from adult patients with gastric cancer and transplantation in severe combined immunodeficiency (SCID) mice.And SCID mice were treated with concentrations of 0.2,0.4,0.8 g · kg-1 octadecenoic acid,respectively.The SCID mice in control group treated with concentration of 100 mg · kg-1 fluorouracil(5-FU).The SCID mice in model group treated with equal amounts of rapeseed oil.The duration was 4 weeks.The cyclic adenosine monophosphate (cAMP),tumor suppressor gene p53 (P53),phosphatidylinositol 3-kinase(P13K) levels in tumor tissue were detected by the method of enzyme linked irnmunosorbent assay.Results Compared to the model group,growth inhibition rate of transplantation tumor of human gastric cancer in low,middle,high experimental groups and control group were increased 26.52％,58.75％,67.80％ and 46.25％ respectively with significace(P ＜ 0.01);the cAMP levels were increased by 35.61％,74.93％,130.48％ and 54.98％ inthe four groups,respectively with significance also (P ＜ 0.01);the P53 and P13K levels were reduced by 57.22％,79.45％,95.71％,80.10％ and 33.11％,44.39％,97.42％,51.69％ respectively in the four groups with significance too(P ＜ 0.01).Conclusion Growth inhibition mechanism of octadecenoic acid on transplantation tumor of human gastric cancer,mainly related to elevated cAMP level within the tumor tissue,inhibit the activity of P53 and P13K signal pathway.
