Protective effect of Shenmai injection on the behavior change in adult rats after neonatal maternal separation
10.13699/j.cnki.1001-6821.2017.04.013
- VernacularTitle:参麦注射液对母婴分离新生鼠脑损伤的保护作用
- Author:
Jia-Wen DANG
1
;
Hong-Yan SUN
;
Wen-Bin DONG
;
Qing-Ping LI
;
Zhi-Qiang FENG
;
Xiao-Ping LEI
Author Information
1. 西南医科大学附属医院新生儿科
- Keywords:
neonatal mother separation;
three links exchange apparatus of brain function;
Shenmai injection;
Bcl-2 protein
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(4):335-337,342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of Shenmai injection (SI)on the behavior change in adult rats after neonatal maternal separation.Methods Neonate-mother separation (NMS) animal model was replicated.The rats in maternal separation group were separated for 3 h per day on postnatal days 2 to 21.Neonatal rats were divided randomly into three groups (n =12):control group,model group (NMS),experimental group (SI).Each group had twelve rats.After two months,the behavior changes of adult rats were tested.The expression of Bcl-2 in hippocampus was detected by immunohistochemical method.Results The total distances in experimental group and model group were (15.17 ± 5.32),(12.32 ± 2.74) m with significant difference (P ＜ 0.05).The average speeds in experimental group and model group were (0.09 ± 0.03),(0.07 ± 0.04) m · s-1 with significant increase (P ＜ 0.05),But not to the same as that of control group.The expression of hippocampus Bcl-2 in model group,control group and experimental group were 110.34 ± 37.32,92.62 ± 52.13,152.17 ± 29.65 with significantly increased in model group compared with control group (P ＜0.05);while decreased in experimental group compared with model group (P ＜0.05).But not to the same as that of control group.Conclusion Shenmai injection possibly can improve the cognitive impairment,which may be related to the upregulation of the expression level of apoptosis gene Bcl-2.
