Effect of cerebral ischemic preconditioning on endogenous injury after cerebral ischemic reperfusion in rats
10.13699/j.cnki.1001-6821.2017.04.012
- VernacularTitle:脑缺血预处理对脑缺血再灌注大鼠内源性损伤的影响
- Author:
Fei MA
1
;
Yao-Yuan ZHANG
;
Yu-An ZOU
;
Qian XUE
;
Li ZHANG
;
Ai-Xia SONG
Author Information
1. 河北北方学院
- Keywords:
cerebral ischemic preconditioning;
cerebral ischemic reperfusion injury;
endogenous injury;
Fas;
FasL;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(4):330-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effects of cerebral ischemic preconditioning on the changes of Fas,FasL,Caspase-3 proteins in infarction tissue after cerebral ischemia reperfusion of rats and to explore its mechanism.Methods The rats were randomly divided into three groups as follows:model Ⅰ group,model Ⅱ group and the sham operated group.Each group had thirty rats.The rats in model Ⅰ were prepared by Zea-Longa'middle cerebral artery occlusion.The right internal carotid artery of rats in model Ⅱ group was blocked transiently 10 minutes at one time.After 3 days,the middle cerebral artery occlusion of rat in model Ⅱ group was used to establish focal cerebral ischemia reperfusion injury model according to the model Ⅰ group.The sham operated group only isolated carotid artery.The neural behavior of rats was evaluated and the volume of cerebral infarction was detected after operation at 6,12,24,48,72 h(each group had six rats).The expressions of Fas,FasL and Caspase-3 protein of rats at different time points after operation were observed by immunohistochemical method.The apoptosis rate of nerve cells were detected by TdT -mediated dUTP nick-end labeling (TUNEL)method.Results At the time of 24 h after operated,the neural function defect point in model Ⅰ group,model Ⅱ group and sham operated group of rats were (2.8 ± 0.8),(1.8 ± 0.8),0 point.The expression of Fas protein positive cells in rats' brain tissue were (35.67 ± 2.16) ％,(22.83 ± 1.71) ％,(14.17 ± 2.32) ％ in that three groups.The expression of FasL protein positive cells in rats' brain tissue were(36.50 ±1.38)％,(22.67 ±2.50)％,(13.33 ±1.21)％ in that three groups.The expression of Caspase-3 protein positive cells in rats' brain tissue were(37.33 ± 1.03)％,(23.17 ± 1.47)％,(11.33 ± 1.37)％.The apoptotic rate in brain tissue of rats were(40.58 ± 1.72)％,(23.25 ± 1.13)％,(11.75 ± 1.37)％ in that three groups.Compared with model Ⅰ group,the neural function defect point of rats or the expression rat of Fas,FasL,Caspase-3 protein and the number of apoptotic nerve cells in model Ⅱ group at the same time all decreased;but the two groups were significantly higher than the sham operated group,(all P ＜ 0.05).Conclusion Cerebral ischemic preconditioning can reduce the expression of Fas,FasL and Caspase-3 proteins.Negative regulation of Fas and FasL pathway after ischemic preconditioning may be an important endogenous mechanism of the resistance to damage.
