Study on antioxidative activity of the extract of corn silk
10.13699/j.cnki.1001-6821.2017.03.019
- VernacularTitle:玉米须提取液抗氧化功能特性的研究
- Author:
Hao GUO
1
;
Hong GUAN
;
Hui-Ling HOU
;
Zhen-Yan LIU
;
Xue CHEN
;
Han LIU
;
Chuan-Gang ZANG
;
Yu-Chao LIU
;
Wen-Qin YANG
Author Information
1. 齐齐哈尔医学院医药科学研究院
- Keywords:
corn silk;
half inhibition rate;
antioxidant activity;
free radical
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(3):258-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the main components and antioxidant activity of the extract of corn silk.Methods The extraction of proteins was determined by the Kjeldahl method,total sugar was determined by the phenol-sulfuric acid assay,reducing sugar by 3,5-dinitrosalicylic acid (DNS) assay,respectively.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis assay (SDS-PAGE) profile was used to show the composition of the protein of the extract of corn silk.The antioxidant activity of the extract of corn silk was evaluated by the ABTS · + radical scavenging rate and oxygen free radical absorption capacity.Results The contents of total sugar,reducing sugar and protein were 51.70％,10.43％ and 21.80％,respectively.SDS-PAGE profile showed that the extract of corn silk contained soluble protein,and its molecular weight was between 9.5 kD and 20 kD.The experimental results showed that the free radical scavenging rate of ABTS · + had reached 70.29％ when the extract concentration was 0.40 mg · mL-1 In the reaction system,the half inhibition rate (IC50) of ABTS free radical scavenging activity was 0.23 mg · mL-1.The total antioxidant activities evaluated by ORAC assay were 3.40 mmol Trolox equivalents · g-1,equivalent to 1/3 of absorption capacity of Vitamin C free radical.Conclusion The extract of corn silk has prominent antioxidant activity.
