Determination the free concentrations of valproic acid in human plasma by HPLC
10.13699/j.cnki.1001-6821.2017.03.017
- VernacularTitle:用HPLC法测定人血浆中丙戊酸钠的游离浓度
- Author:
Wei-Chong DONG
1
;
Jia-Liang GUO
;
Ye JIANG
;
De-Qiang LI
;
Zhi-Qing ZHANG
Author Information
1. 河北医科大学第二医院药学部
- Keywords:
valproic acid;
free concentration;
hollow fiber;
centrifugal ultrafiltration;
high performance liquid chromatography
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(3):251-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a hollow fiber centrifugal ultrafiltration-high performance liquid chromatography (HPLC) method for the analysis of free concentration of valproic acid (VPA) in human plasma.Methods The plasma samples were prepared by hollow fiber centrifugal ultrafiltration,and then were derivated by 2-bromo-2-acetonaphthone before HPLC analysis.Diamonsil RP-C1scolumn (150 mm × 4.6 mm,5 pμm) was used.The mobile phase consisted of methanol and water (80:20) at the flow rate of 1.0 mL · min-1 The column temperature was maintained at 30 ℃.The detected wavelength was 251 nm.The specificity,linearity,the limit of detection (LOD),precession,recovery and stability of the developed method were validated.Results The good linear relationship was obtained between the free concentration of VPA from 0.05-50.00 μg · mL-1 (r =0.999 0) with no significant interference from endogenous compounds.The limit of detection for the analysis of free VPA was 0.01 μg · mL-1.The method recovery rates of free VPA were 96.51％-101.22％ The absolute recovery rates were 94.28％-99.15％,respectively.The intra-day and inter-day precision (RSD) were all less than 4.00％.The VPA was all stability at the conditions of maintaining at room temperature for 10 h,after freeze-thaw cycle and keeping at-80 ℃ for 21 days.Conclusion The present method is simple,accurate and sensitive,which is suitable for the analysis of free concentration of VPA in human plasma.
