Induce effect of iodinated contrast media on necrosis of human renal tubular epithelial cell
10.13699/j.cnki.1001-6821.2017.03.016
- VernacularTitle:含碘造影剂对人肾小管上皮细胞坏死的诱导作用
- Author:
Xiao-Feng GUAN
1
;
Qing-Jie CHEN
;
Wen-Jun YIN
;
Dai-Yang LI
;
Jiang-Lin WANG
;
Xiao-Cong ZUO
Author Information
1. 中南大学湘雅三医院药学部
- Keywords:
contrast media;
renal tubular epithelial cell;
necrosis;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(3):248-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effection necrosis of human renal tubular epithelial (HK-2) cell induced by iodinated contrast media.Methods The HK-2 cell was cultured in vitro.The experiment was divided into three groups:control group,iohexol group,urografin group.HK-2 cell was treated with iohexol(75 mgI · mL-1) and urografin(75 mgI · mL-1).Cell vitality was determined by MrTF assay at time of 2 h after intervention and of 5,22 h after drug removal.The release of lactate dehydrogenase (LDH) in liquid supernatant was detected by using LDH kit.Quantitative analysis of Hoechst33342/PI staining results was carried out by high content imaging system.Apoptosis rate and death rate were calculated.Results Compared with the control group,iohexol and urografin induced the cell vitality decreased to (52.98 ± 6.75)％,(47.16 ±4.18)％ at time of 2 h after intervention with statistically significant (P ＜ 0.01).And the release of LDH increased to (201.31 ± 11.69),(256.21 ± 19.82) U · L-1 with statistically significant (P ＜0.01).This effect still existed at 5 h and 22 h after the drugs removed.The apoptosis rates of iohexol and urografin were (23.09 ± 4.25) ％,(38.64 ± 8.32) ％;the necrosis rates of iohexol and urografin were(19.69 ± 2.13) ％,(24.07 ± 6.18) ％,which increased significantly compared with the control group (P ＜ 0.01).Moreover,urografin induced HK-2 cell necrosis more significantly (P ＜ 0.05).Conclusion Iodinated contrast media can induce necrosis of human renal tubular epithelial cell.
