Determination of imatinib in human plasma by UPLC-MS/MS

VernacularTitle:超高效液相色谱－质谱法测定人血浆中伊马替尼的浓度
Author: Wei ZHANG ¹ ; Ji DONG ; Li-Yan MIAO
Author Information

1. 苏州大学附属第一医院药学部
Keywords: imatinib; UPLC -MS/MS; therapeutic drug monitor; plasma
From: The Chinese Journal of Clinical Pharmacology 2016;32(14):1329-1331
CountryChina
Language:Chinese
Abstract: Objective To establish the UPLC-MS/MS method for the determination of imatinib in human plasma . Methods The plasma procedure involved a single -step protein precipitation by acetonitrile . The samples were separated by a ACQUITY UPLC BEH C 18 column (100 mm ×2.1 mm, 1.7 μm) using a mobile phase consisted of acetonitrile-0.1%formic acid ( 25:75 ) at a flow rate of 0.4 mL? min-1 .The protonated ions of analytes were detected in positive ionization in multiple reaction monitoring mode ( MRM ) . The specificity , standard curve , lower limit of quantitation , precision , recovery rate and stability as well as the matrix effect were investigated.Results The calibration curve was linear over a concentration range of 0.1 to 20.0 μg? mL-1 ( r=0.996 ) and the low limit of quantitation was 0.1 μg? mL-1 .The extraction recovery rates were ranged from 95.25% to 98.21%, with-day RSD and between -day RSD were all less than 15%. Conclusion The method is simple and quick , with high specificity and sensitivity, and suitable for clinical therapeutic drug monitoring of imatinib.