Effect of carnosol on chemokines expression in tumor necrosis factor-α-induced HT-29 cells
10.13699/j.cnki.1001-6821.2016.14.022
- VernacularTitle:鼠尾草酚对肿瘤坏死因子－α诱导 HT －29细胞趋化因子表达的影响
- Author:
Hui YAO
1
;
Dong XIA
;
Ming-Ming WANG
;
Wang-Sheng CHEN
;
Lei REN
;
Liang XU
Author Information
1. 西南医科大学 附属医院 胃肠外科
- Keywords:
carnosol;
tumor necrosis factor-α;
chemokine;
nuclear factor-κB
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(14):1325-1328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of carnosol on chemokines ex-pression in tumor necrosis factor -α( TNF-α)-induced HT-29 cells and its mechanism.Methods Cells were divided into control group ( DMEM medium ) , TNF -αgroup ( 10 ng? mL-1 TNF -α) , low, medium, high-dose carnosol groups (10 ng? mL-1 TNF -α+10, 20 , 40 μmol? L-1 carnosol ) and positive control group ( 10 ng? mL-1 TNF-α+40 μmol? L-1 ammonium pyrrolidinedithiocarbamate ).Cell counting kit-8 ( CCK-8 ) were used for analysis of HT -29 viability. The expression of interleukin -8 ( IL-8 ) and monocyte chemoattractant protein-1 ( MCP-1 ) were examined by enzyme linked immunosorbent assay.The expression of nuclear factor -κB ( NF -κB ) p65 were evaluated by Western -blot.Results The optical densities were (1.21 ±0.04 ), (1.17 ±0.06 ), (1.16 ±0.04 ), (1.15 ±0.05), (1.15 ±0.05),(1.18 ±0.05) in control, TNF-α, low-dose, medium-dose, high-dose and positive control group , also , there were no significant differences on cell viability in six groups ( P>0.05 ) .The ex-pressions of IL-8 and MCP-1 were (4942.00 ±220.30 ), (2299.00 ±273.38 )pg? mL-1 in TNF-αgroup, which were significantly higher than those in control group [ ( 841.67 ±27.15 ) , ( 140.67 ±23.54 ) pg? mL-1 , P<0.05 ] . Among the low -dose, medium -dose, high -dose and positive control group , the expressions of IL -8 were ( 4136.33 ±253.00 ) , ( 3156.00 ±241.89 ) , ( 2585.67 ±185.04 ) , ( 2652.67 ±219.55 ) pg? mL-1 and MCP -1 were (1980.67 ±167.02), (1259.33 ±256.66), (851.00 ±161.16), (1452.00 ±256.66)pg? mL-1, which were significantly lower than those in TNF -αgroup.The expression of NF-κB p65 in low, medium, high-dose carnosol groups were significantly lower than those of TNF -αgroup ( P <0.05 ) .Conclusion Carnosol can inhibit the expression of IL-8 and MCP-1 in TNF-α-induced HT-29 cells, which may associate with inhibition of TNF-α-induced NF-κB pathway activation.
