Relationship between micro RNA542 -3 p and the invasion and metastasis for colorectal cancer cell
10.13699/j.cnki.1001-6821.2016.14.020
- VernacularTitle:微小 RNA542－3p与结直肠癌细胞侵袭转移能力的相关性研究
- Author:
Kun-Hou YAO
1
;
Yong-Ping MA
;
Jun-Hong HU
;
Ji-Ming MENG
Author Information
1. 河南大学淮河医院普通外科
- Keywords:
colorectal cancer;
micro RNA542-3 p;
invasion and metastasis;
cell line
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(14):1318-1321
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the molecular mechanism of micro RNA542-3p (miR-542-3p) in the invasion and metastasis of colorec-tal cancer.Methods Forty paired colorectal cancer patients with cancer tissue and relative normal colorectal tissue , different metastasis ability colorectal cancer cell lines ( Lovo, LS1747, SW620, HT29, SW480 ) and normal colorectal epithelial cells were tested of the miR-542-3 p expression by real -time polymerase chain reaction.The ability of prolif-eration or migration for SW480 or HT29 cells were tested after transinfec-tion of miR-542-3p, mimics and inhibitor after 24 h.The physiologi-cal characteristics such as proiferation , migration of colon cancer cells was evaluated.The biological function of miR -542-3 p was evaluated in colorectal cancer cells.Results MiR-542-3p expression in cancer tissues was significantly lower than that in normal intestinal epithelium ( P<0.05 ) .MiR-542-3 p was higher expressed in normal colorectal epithelial cells compared to colorectal cancer cell lines ( HT29 , SW620 and Lovo , P<0.05 ) .MiR-542-3 p was higher expressed in the high metastasis cancer cell lines ( SW620 and Lovo ) compared to low metastasis cancer cells (P<0.05).After 24, 48, 72 and 96 h, the cell proliferation ability was no significant difference in inhibitor mir-542-3p inhibitor group, miR-542-3p mimics group, control group, mimics control group (P>0.05).After 24 h, the migration ability in mir -542-3p mimics group was significantly lower than that of mimics control group ( P<0.05 ) , and the migration ability in miR -542-3 p inhibitor group was significantly higher than that of inhibitor control group (P<0.05).Conclusion MiR-542-3p may play a role in inhibiting cell metastasis in colon cancer cells, which can be used as biomarker for colorectal cancer metastasis .
