Effect of calcium dobesilate on the apoptosis of retinal cells in diabetic rats
10.13699/j.cnki.1001-6821.2016.14.019
- VernacularTitle:羟苯磺酸钙对糖尿病大鼠视网膜细胞凋亡的影响
- Author:
Li FA
1
;
Cun-Wen PEI
;
Yue-Xi JIN
Author Information
1. 承德市中心医院 眼科
- Keywords:
calcium dobesilate;
diabetic retinopathy;
cell apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(14):1314-1317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of calcium dobesilate on the apoptosis of retinal cells in diabetic rats .Methods Thirty-six Wistar rats were randomly into three groups:normal group ( n =12 ) , model group(n=12), test group(n=12).The rat in model and test groups of diabetes was given with high fat diet for 8 weeks, and then was made by intraperitoneal injection of streptozotocin ( STZ ) .After successfully es-tablished the diatetes animal model , the rats in test group was lavaged with 150 mg? kg -1? d-1 calcium dobesilate continuous 8 weeks, the rats in normal and model group were given same amount of normal saline . Neuronal apoptosis in retina was detected by TdT -mediated dUTP nick end labeling ( TUNEL ) . The expression of B -cell lymphoma -2 ( Bcl-2 ) , Bcl -2 assaciated X protein ( Bax ) and p53 protein and mRNA were assayed by Western blot and reverse transcription -polymer-ase chain reaction.The activity of cysteinyl aspartate specific proteinase 3 ( Caspase 3) was measured by spectrophotometry .Results Compared with normal group , apoptotic index of retina , the expression of Bax , p53 protein and mRNA , the activity of Caspase 3 was increased ( P<0.01 ) , the expression of Bcl -2 protein and mRNA was decreased in model group ( P<0.01 ) . The expression of Bax , Bcl -2 and p53 mRNA was ( 0.20 ±0.03 ) vs ( 1.00 ±0.09 ) , ( 1.39 ±0.10 ) vs (0.21 ±0.02);(0.28 ±0.05) vs (0.88 ±0.08), in normal group and model group, respectively.Compared with model group,apoptotic index of retina (12.40 ±1.24), the expression of Bax (0.57 ±0.05) and p53 (0.35 ±0.03) protein, the expression of Bax ( 0.56 ±0.05 ) and p53 ( 0.36 ±0.04 ) mRNA, the activity of Caspase 3 (1.72 ±0.17) in test group were all decreased.While the expression of Bcl -2 protein (0.63 ±0.06) and mRNA (0.74 ±0.04 ) were increased in test group ( all P<0.01 ) .Conclusion The calcium dobesilate inhibit neuronal apoptosis by regulation expression of cell apoptotic related protein .
