Determination of (S) -MP 3950 in rat plasma by ultra performance liquid chromatography - tandem mass spectrometry and its application to a pharmacokinetic study
10.13699/j.cnki.1001-6821.2016.13.022
- VernacularTitle:超高效液相串联质谱法测定大鼠血浆中（S）－MP 3950的浓度及其药代动力学研究
- Author:
Cheng-Ying WANG
1
;
Hong SUN
;
Feng QIN
;
Shao-Jie WANG
;
Zhong-Gui HE
;
Long-Shan ZHAO
;
Zhi-Li XIONG
Author Information
1. 沈阳药科大学 药学院
- Keywords:
( S)-MP 3950;
ultra performance liquid chromatography -tandem mass spectrometry;
pharmacokinetics
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(13):1227-1230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an ultra performance liquid chromatog-raphy-tandem mass spectrometry ( UPLC -MS/MS ) method for the study of pharmacokinetics of ( S ) -MP 3950 , a novel gastroprokinetic agent candidate in rats.Methods The plasma samples were extracted by liquid -liquid extraction ( LLE ) with ethyl acetate.An ACQUITY UPLC ? BEH C18 column (2.1 mm ×50 mm, 1.7μm) was used with a mobile phase consisting methanol and 5 mmol? L-1 ammonium acetate with 0.1% formic acid ( v∶v=45∶55 ) .The analysis was performed in multiple reaction monitoring(MRM) mode via positive electrospray ioni-zation source on a triple quadrupole tandem mass spectrometer . The whole analysis time was 3.0 min.The pharmacokinetic parameters were calculated by DAS 2.0 program.Results The method had a lower limit of quantification(LLOQ) of 10 μg? L-1, linear up to 5000 μg? L-1. The intra-and inter-day precision(relative standard deviation, RSD) were all less than 7.9%.The accuracy ( relative error , RE ) was from 0.1%to 8.5%.Conclusion The method was proved to be rapid , sen-sitive and accurate for pharmacokinetic study of ( S)-MP 3950 in rats.
