Effect of silibinin on the lipid of cellular model with non -alcoholic fatty liver disease
10.13699/j.cnki.1001-6821.2016.13.015
- VernacularTitle:水飞蓟宾对非酒精性脂肪肝模型脂代谢的影响
- Author:
Dong YAN
1
;
Xue-Fei WANG
;
Xiu-Chao SUN
;
Tao JIANG
;
Yuan-Yuan WEI
Author Information
1. 新疆医科大学 基础医学院 药理学教研室
- Keywords:
silibinin;
non-alcoholic fatty liver disease;
tumor necrosis factor-α
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(13):1202-1205
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of silibinin on the lipid metabolism of cellular model of non -alcoholic fatty liver disease (NAFLD) induced by oleic acid.Methods The cellular models of NAFLD was divided into normal group , model group ( OA 0.2 mmol? L-1 ) , high dosage , moderate dosage and low dosage test groups ( OA 0.2 mmol? L-1 +silibinin 2.5,5.0,10.0μg? mL-1 , respectively ) . The cellular levels of triglyceride ( TG) were determined by enzyme cou-pling chromometry.The superoxide dismutase ( SOD) were measured by xanthine oxidase reaction.The malonaldehyde ( MDA) were evaluated by thiobarbituric acid method.The glutathione peroxidase ( GSH-PX) were determined by enzymatic method . The tumor necrosis factor -α(TNF-α) were detected by ELISA. The adenosine monophosphate activated protein kinase ( AMPK ) , Sirtuin -3 ( SirT3 ) , acetyl -CoA carboxylase 2 ( ACC 2 ) and carnitine palmitoyltransferase -1 A ( CPT-1A) mRNA expression were quantitatively determined by real -time quantitative PCR ( RT -PCR ) .The intracellular lipid acceleration was observed by oil red O stain . Results Compared to the model group on TG level with ( 500.05 ±152.01 ) mg? g-1 , TG level in high dosage test group with (164.74 ±53.94) mg? g -1 decreased significantly(P<0.01).Compared to the model group on SOD level with ( 4.96 ±1.39 ) U? mg -1 ,the SOD level in high dosage test group with ( 20.37 ±11.16 ) U? mg -1 was increased significantly ( P<0.01 ) .Compared to the model group on MDA and TNF -αlevels with ( 0.20 ±0.03 ) U? mg -1 , (32.03 ±8.04 ) pg? mL-1 , MDA and TNF -αlevels in high dosage test group with ( 0.10 ±0.02 ) U? mg -1 , (15.00 ±0.53)pg? mL-1 were decreased significantly(P<0.01, P<0.05).Compared to the model group, Ampk, SirT3, CPT-1A mRNA expression levels in high dosage test group were much higher (P<0.01), but ACC2 mRNA expression level was much lower ( P <0.01 ) .Conclusion The TG level of cellular model of NAFLD could be decreased effectively by silibinin , which might be related to its antioxidant activity and regulation of lipid metabolism related gene expression levels .
