Study on the pharmacokinetics and cardiac distribution of tetramethylpyrazine hydrochloride in mice
10.13699/j.cnki.1001-6821.2016.13.013
- VernacularTitle:盐酸川芎嗪在小鼠体内的药代动力学和心脏分布研究
- Author:
Rui-Fang LI
1
;
Yang GAO
;
Xiang-Jun QIU
;
Ya-Nan PENG
;
Jun-Min FU
;
Hong-Yun WANG
Author Information
1. 河南科技大学 医学院 药理学与分子生物学重点实验室
- Keywords:
tetramethylpyrazine hydrochloride;
RP-HPLC;
pharmaco-kinetics;
cardiac distribution
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(13):1194-1197
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pharmacokinetics and cardiac distribution of tetramethylpyrazine ( TMP ) hydrochloride in mice by re-versed phase high performance liquid chromatography ( RP -HPLC ) method.Methods The plasma and heart homogenate of mice were trea-ted with acetonitrile and a RP -HPLC method was developed.The 66 mice were injected intraperitoneally with a single dose of 80 mg? kg -1 . The concentration of TMP in plasma and heart was determined .The pa-rameters of the pharmacokinetics were calculated .Results This RP-HPLC method had a good linearity , recovery rate and precision.The standard curve of TMP was linear in the range of 0.2-20.0 mg? L-1 ( r=0.999 7 ).The major parameters of TMP were as follow: AUC0-t was (671.20 ±31.49) mg? min? L-1,Cmax was(17.54 ±0.71) mg? L-1, tmax was 10 min, t1/2 was (25.47 ±14.85 ) min.Conclusion A sim-ple, accurate and sensitivity RP-HPLC method is developed and valida-ted for the determination of TMP concentration in plasma and heart of mice.TMP has a rapid absorption and higher concentration in the heart , which was beneficial for the treatment of heart disease .
