Effects of berberine compatible with stachyose on glucolipid metabolism and gut microbiota in diabetic mice
10.13699/j.cnki.1001-6821.2016.12.021
- VernacularTitle:小檗碱配伍水苏糖对糖尿病小鼠糖脂代谢及肠道菌群的影响
- Author:
Yu-Wei HAN
1
;
Cai-Na LI
;
Yi HUAN
;
Su-Juan SUN
;
Yong-Zhao MU
;
Zhu-Fang SHEN
Author Information
1. 中国医学科学院 北京协和医学院 药物研究所天然药物活性物质与功能国家重点实验室
- Keywords:
berberine;
stachyose;
glucolipid metabolism;
gut microbiota
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(12):1121-1124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of berberine compatible with stachyose on glucolipid metabolism and gut microbiota in type 2 dia-betic KKAy mice.Methods Female diabetic KKAy mice selected by insulin tolerance test (ITT) were divided into control group ( water 0.1 mL· 10 g -1 ), berberine group ( berberine 100 mg · kg -1 · d-1 ), stachyose group ( stachyose 200 mg · kg -1 · d-1 ) and combined group ( berberine 100 mg· kg-1 · d -1 +stachyose 200 mg · kg -1 · d -1 ) , 9 mice per group.Ten female C57BL/6J mice were served as normal con-trol group ( water 0.1 mL· 10 g -1 ).All mices were orally administrated once daily for 7 weeks.Fasting plasma glucose and lipids levels were detected.Oral glucose tolerance test ( OGTT) and ITT were determined at the end of treatment.In addition, the quantities of Lactobacillus and Bifidobacterium in mices intestine were examined by 16 SrRNA-targeted real -time fluorescence quantitative polymerase chain reaction.Results Compared with control group , combined group was markedly improved glucose tolerance and insulin sensitivity ( P<0.01 ) , decreased fasting plasma glucose and total cholesterol levels ( P <0.05 ) and enriched the abundance of Lactobacillus ( P<0.01 ) and Bifidobacterium ( P<0.01 ) , which were significantly better than berberine group and stachyose group.Conclusion Berberine combined with stachyose provided a better control for glucolipid metabolism than any single component in diabetic KKAy mice , which was related to up -regulation of Lactobacillus and Bifidobacterium in mice intestine.
