Inhibition effects of erlotinib on invasion and metastasis in non -small cell lung cancer brain metastasis cell PC14/B
10.13699/j.cnki.1001-6821.2016.12.019
- VernacularTitle:厄洛替尼对非小细胞肺癌脑转移细胞 PC14／B侵袭转移能力的抑制作用
- Author:
Chan-Chan HU
1
;
Xiang-Yao LIAN
;
Cui-Min ZHU
;
Ai-Ke LI
;
Yun-Wei LIANG
;
Qing-Shan LI
;
Rui WANG
Author Information
1. 承德医学院 附属医院 肿瘤科
- Keywords:
erlotinib;
non-small cell lung cancer brain metastasis cell PC14/B;
invasion;
metastasis
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(12):1118-1120
- CountryChina
- Language:Chinese
-
Abstract:
Obejective To explore the inhibition effects of erlotinib on invasion and metastasis in non -small cell lung cancer brain metastasis cell PC14/B.Methods MTT method was used to detect the viability of PC14/B cells 48 h after the cultivation with erlotinib of 0 ( control group), 1, 5 and 25 μmol· L -1(erlotinib ,test groups).The cell inva-sion was detected by tranaswell method.The phosphorylation of protein kinase B ( AKT ) and the expression of matrix metalloproteinase 2 ( MMP2 ) , MMP9 epithelial -mesenchymal transition ( EMT ) related proteins including E -cadhherin and Vimentin were detected by Western blot.Results Compared with control group (0.75 ±0.09), three dose test groups[(0.63 ±0.06), (0.52 ±0.04), (0.38 ±0.03)] inhibited the cell viability.Compared with control group , three dose test groups inhibited invasion, up -regulated the expression of E -cadherin, and down-regulated the expressions of MMP2, MMP9, Vimentin and the phosphorylation of AKT.Conclusion Erlotinib suppressed PC 14/B cell invasion and metastasis , which was related to the down -regulation of the expression of MMPs , and the inhibition on the generation of EMT and the phosphorylation of AKT.
