Effect of propofol on the apoptosis of PC12 cell induced by glutamic acid
10.13699/j.cnki.1001-6821.2016.12.018
- VernacularTitle:丙泊酚对谷氨酸诱导的 PC12细胞凋亡的影响
- Author:
Zheng LI
1
;
Jin-Chun SI
;
Xiang-Yu GAO
;
Zhe LIU
;
Nan LIANG
;
Zheng NAN
Author Information
1. 河南省南阳市中心医院 手术二部
- Keywords:
propofol;
PC12 cell;
cell apoptosis;
FBJ osteosarcoma oncogene;
early growth response protein -1
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(12):1115-1117
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of propofol on the apoptosis of PC12 cell induced by glutamic acid.Methods PC12 cells were in-duced 10 mmol · L-1 glutamate for 48 h, and were divided into model group , propofol low , medium and high dose groups.The normal cells were used as control group.Control group and model group were received culture medium without any drugs for 48 h.Propofol low , medium and high dose groups were given 12.5 , 25.0 , 50.0 μmol · L-1 propofol for 48 h.The viability of PC12 cell was measured by MTT assay.PC12 cell apoptosis was measured by flow cytometry.The activity of Caspase -3 was determined by spectrophotometric method.The expression of FBJ osteosarcoma oncogene ( c -fos ) and early growth response protein 1 ( Egr -1 ) were detected by Realtime -PCR and western blot.Results Compared with the normal group , the cell apoptosis rate , the activity of Caspase -3 , the expression of c -fos mRNA and protein increased , and the cell viability , the expression of Egr -1 mRNA and protein decreased in the model group ( P<0.01 ).Compared with the model group, the acitivity of Caspase -3, the cell apoptosis rate , the activity of Caspase -3, the expression of c-fos mRNA and protein decreased , and the cell viability , the expression of Egr-1 mRNA and protein increased in propofol low -dose , medium -dose and high -dose group ( P <0.05 ).Conclusion Propofol suppressed the apoptosis of PC 12 cell induced by glutamic acid , which was related with the expression of c-fos and Egr-1.
