Effects of arctigenin on inhibiting proliferation of type Ⅱ endometrial carcinoma cells
10.13699/j.cnki.1001-6821.2016.12.017
- VernacularTitle:牛蒡子苷元对人Ⅱ型子宫内膜癌细胞增殖抑制的研究
- Author:
Jun-Juan XU
1
;
Ya-Fen QIU
;
Yan FENG
Author Information
1. 杭州师范大学 附属医院 妇产科
- Keywords:
arctigenin;
type Ⅱendometrial cancer;
cell proliferation;
vascular endothelial growth factor
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(12):1112-1114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of arctigenin on inhibiting proliferation of type Ⅱ endometrial carcinoma cells , and its relationship with vascular endothelial growth factor ( VEGF).Methods The typeⅡendometrial carcinoma cells line were divided into control group , test A group and test B group.The control group were cultured for 7 days without any drugs.The test A group and test B group were treated with Arctigenin for 7 days at the concentration of 10 μmol · L-1 and 20 μmol· L-1 respectively.Cell proliferation and inhibition level were measured by cell counting kit ( CCK -8 ) and cell growth curve.The expression of mRNA and protein for VEGF were measured by reverse transcription polymerase chain reaction and Western blot.Results Comparing with the control group , the test A group and test B group have inhibitory effects on proliferation of type Ⅱendometrial carci-noma cell line ( P<0.05 ).The effect of inhibition in test B group was more obvious than in test A group ( P<0.01 ).The expression of mRNA and protein for VEGF in test A group and test B group decreased ( P<0.05 ).The expression level in test B group decreased more significantly than in test A group ( P<0.01 ).Conclusion The effect of Arctigenin on inhibiting proliferation of typeⅡendometrial carcinoma cells is implemented by down-regulating the genetic expression of VEGF.
