Effects of cobalt chloride on the expressions of stromal cell derived factor 1 and chemokine receptor 4 in cerebral ischemia rats
10.13699/j.cnki.1001-6821.2016.12.016
- VernacularTitle:氯化钴对大鼠脑缺血模型基质细胞衍生因子－1及趋化因子受体4表达的影响
- Author:
Jia LI
1
;
Chang-Lin ZOU
;
Zhi-Ming ZHANG
;
Chun-Yang ZHANG
Author Information
1. 天津中医药大学 附属南开医院
- Keywords:
cobalt chloride;
cerebral ischemia;
stromal cell derived factor -1;
chemokine receptor;
pretreatment
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(12):1109-1111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of cobalt chloride precondi-tioning on the expression of stromal cell derived factor ( SDF -1 ) and CXC family chemokine receptor 4 ( CXCR4) protein in rats with cerebral ischemia .Methods The 45 SD rats were randomly divided into sham group with 15 rats, model group with 15 rats, and test group with 15 rats.For model group and test group , cerebral ischemia model was estab-lished with suture emboli method.The rats in test group before modeling 2 d, 1 d were given intraperitoneal injection of cobalt chloride ( 30 mg· kg -1 ).Western blotting was used to detect the expression of SDF -1, CXCR4 protein.Apoptosis rate of rat brain cells in each group was tested by Terminal deoxynucleotidyl transferase -mediated dUTP-biotin nick end labeling assay ( TUNEL ).And after 2 days, calculate brain tissue necrosis area of the rats in model group and test group by staining with triphenyl tetrazolium chloride ( TTC ).Results Compared with sham group ( SDF-1 , CXCR4 protein is very low ) , the expression of SDF-1, CXCR4 proteins in the test group and model group increased at each time point ( P<0.05 ) .That was suggested that the expression of CXCR4 and SDF-1 could be up-regulated by cobalt chloride.At time of 2 d after model established , brain cell apoptosis rate in test group was significantly lower than that in model group [ (9.31 ±0.79 )%vs (18.99 ±1.13 )%, P<0.05 ].The brain infarct area in test group was significantly lower than that in model group ( P<0.05 ).Conclusion Chromium chloride can increase the expression of SDF -1, CXCR4 proteins, reduce the brain cell apoptosis rate , and narrow the area of necrosis of cerebral ischemia , which has a protec-tive effect for the ischemic brain tissue.
