Quantification of estradiol in human plasma by liquid chromatography -tandem quadrupole mass spectrometry
10.13699/j.cnki.1001-6821.2016.08.019
- VernacularTitle:液相色谱法串联质谱法测定人血浆中雌二醇含量
- Author:
Long-Mei CHENG
1
;
Guang-Tao HAO
;
Xue-Yi CHEN
;
Ying-Fu ZHANG
;
Rui-Hua DONG
;
Ze-Yuan LIU
;
Heng-Yan QU
Author Information
1. 军事医学科学院 附属医院 临床药理学研究室
- Keywords:
estradiol;
liquid chromatography-tandem quadrupole mass spectrometry;
plasma;
dansyl chloride;
derivation
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(8):733-735,761
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a simple and highly sensitive liquid chromatography-tandem quadrupole mass spectrometry method for deter-mination of estradiol in human plasma.Method Estradiol was extrac-ted using liquid-liquid extraction method from human plasma, with est-rodial-d3 as an internal standard.After extracted, samples were deri-vatizated by dansyl chloride in alkaline conditions.The Atlantis T3 (2.1 mm ×100 mm, 5.0 μm) column was used for separation.The gradient mobile phase was 0.1% formic acid and acetonitrile with a flow rate of 0.5 mL? min-1 , the temperature was set at 30 ℃, and the injection volume was 10 μL.The detection was performed with multiple reactions monitoring using positive electrospray ionization with API5500 as the detec-tor.Results The linear ranges of estradiol was 1 -100 pg? mL-1 , lower limit of quantification was 1 pg? mL-1 , the regression equation was y=5.47 ×10 -2 x+4.67 ×10 -2 .The inter-and intra-day RSD were less than 15%and the absolute recovers were above 97%.Estrodiol was proved to be stable under study station.Conclusion This method is specific, sensitive, accurate, and suitable for the pharmacokinetic study of estradiol in human plasma.
