Methyladenine regulation of autophagy to promote endothelial progenitor cells in rats and its mechanism of venous thrombosis recanalization
10.13699/j.cnki.1001-6821.2016.08.015
- VernacularTitle:3－甲基腺嘌呤调控自噬对大鼠内皮祖细胞促进静脉血栓再通的作用及其机制
- Author:
Guang-Wei YANG
1
;
Jin-Song JIANG
;
Ji-Fu LAI
;
Wei-Qin LU
;
Kai-Ping LU
Author Information
1. 浙江省人民医院血管外科
- Keywords:
methyladenine;
regulation of autophagy;
endothelial progenitor cell;
venous thrombosis
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(8):720-722
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulation of autophagy methylade-nine on promoting the role of endothelial progenitor cells in rats and mechanism of venous thrombosis recanalization.Methods Eighty SD rats were established in the model of venous thrombosis, and divided into four groups, each group had 20 rats.The control group was injected 1 mL concentration of 5 mmol? L-1 of methyl adenine(3-MA) ,the blank group was injected a simple 1 mL saline,the alone group was injected 1 mL endothelial progenitor cells ( EPCs) suspension,the combination group was injected 0.5 mL concentration of 5 mmol? L-1 3-MA and 0.5 mL EPCs suspension.The samples were taken at four time points of 0, 7, 14 and 28 d after injection.Compared with each group of rats, the number of capillary vessels was counted.And the protein expression of vascular endothelial growth factor ( VEGF ) , based fibroblast growth factor ( bFGF) , angiopoietin1 ( Ang -1 ) , membrane cofactor protein 1 ( MCP-1) were measured by Western blot method.Changes of protein expression in 4 groups was compaered.Results The cross-section of the lumen in blank group was more than the control group and the alone group after administration and the samples of the structure was the most in combined group. The capillary number in control group was significantly higher than that in the blank group and the alone group ( P<0.05) , while the capillary number in the combined group was significantly higher than that in the other 3 groups (P<0.05).After twenty-eighth days, the protein expression of VEGF, bFGF, MCP-1 and Ang-1 in combined group was significantly higher than that in other three groups ( P<0.05).Among them, the control group, single use group, combined group and blank group of VEGF were ( 0.51 +0.24 ) , ( 0.62 +0.14 ) , ( 2.04 +0.15 ) , (0.89+0.28)pg?mL-1,respectively.Conclusion 3-MAcanbeproperlyregulatedautophagy,canimprove thrombus local EPCs proliferation and capillary formation and thrombus and recanalization.
