Protective effect of curcumin preconditioning on neurologic function in mice with acute spinal cord injury and its correlation with transforming growth factor -activated kinase 1 pathway
10.13699/j.cnki.1001-6821.2016.08.013
- VernacularTitle:姜黄素预处理保护急性脊髓损伤小鼠神经功能与转化生长因子激酶1的相关性研究
- Author:
Gui-Cai WEI
1
;
Nan-Wen ZHANG
;
Yun-Jiao HE
;
Jin-Tian WANG
;
Yan-Liang CAI
;
Jian-Qing CHEN
;
Zeng-Kai WU
Author Information
1. 福建医科大学 附属南平第一医院 骨科
- Keywords:
acute spinal cord injury;
curcumin;
oxidative stress;
inflammation;
transforming growth factor-activated kinase 1
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(8):714-716
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of curcumin pre-conditioning on neurologic function in acute spinal cord model ( SCI ) mice and its possible mechanism.Methods Divide healthy and clean class mice into sham-operated group,model group,low-dose, middle-dose and high-dose test groups ( curcumin, 50, 100, 200 mg? kg-1 ).Each mouse respectively was given intraperitoneal injection of drugs at 30 min before the surgery.Then, establish experimental animal models following improved Allen′s model. The behavior evaluation was performed on mice.The transforming growth factor -activated kinase 1 ( TAK1 ) , p-TAK1 , MKK6 , p -MKK6 , p38 and p -p38 antibody expression were examined with Western blot.Results Compared with the model group (0.70 ±0.48), after 48 h SCI, the Basso mouse scale (BMS) scores in test group from low to high dose ( 0.80 ± 0.79 ) , (1.20 ±0.63), (1.60 ±0.52) were improved with different degrees. The curcumin markedly inhibited various inflammatory mediators induced by SCI-induced.Curcumin also significantly down-regulated the phos-phorylation levels of TAK1, mitogen-activated protein kinase kinase 6 ( MKK6) and p38 MAPKs, which played key roles in microglia-media-ted inflammatory response. Curcumin could significantly improve the functional recovery compared to the mice without curcumin treatment ( BMS assay ) .Conclusion The preliminary study shows that the way of curcumin preconditioning to improve neurologic function may via TAK1/( MKK6)/p38 pathway.
