Determination of milnacipran in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2016.07.021
- VernacularTitle:高效液相色谱－质谱联用法测定人血浆中米那普仑的质量浓度研究
- Author:
Xue-Yi CHEN
1
;
Guang-Tao HAO
;
Ying-Fu ZHANG
;
Long-Mei CHENG
;
Rui-Hua DONG
;
Yu-Guang LIANG
;
Xiao-Fang WANG
;
Yuan-Yuan LI
;
Ze-Yuan LIU
;
Heng-Yan QU
Author Information
1. 军事医学科学院 附属医院 临床药理室
- Keywords:
HPLC-MS/MS;
milnacipran;
plasma concentration
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(7):643-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC-MS/MS method for s deter-mination of milnacipran in human plasma.Methods The plasma sam-ples were prepared by protein precipitation.The separation was achieved on ZORBAX SB-Aq column(2.1 mm ×50 mm,3.5μm), eluted with a linear gradient using methanol -0.01% formic acid at the flow rate of 0.4 mL· min -1.The column temperature was 30 ℃.Detection of the analyte was achieved by positive ion electrospray ionization in the multi-ple reaction monitoring ( MRM ) mode.The specificity, lower limit of quantitation and standard curve, precision and recovery rate and stability as well as the matrix effect were investigated.Results The concentration of milnacipran was ranged from 20.0 to 5 00.0 ng · mL-1 ( r>0.999 8 ) , with the lower limit of quantitation of 2.0 ng · mL-1.Intra -day and inter-day relative standard deviations ( RSD ) were both below 15%.The recoveries of low, middle and high concentrations were >96%. Conclusion The method is simple, rapid, sensitive and accurate, specific, suitable for determination of milnacipran in human plasma.
