Comparison of liquid chromatography -tandem mass spectrometry and enzyme multiplied immunoassay technique for the determination of plasma concentration of digoxin
10.13699/j.cnki.1001-6821.2016.07.020
- VernacularTitle:液相色谱－质谱法和酶扩大免疫法测定地高辛血药浓度的方法研究
- Author:
Zhan-Jun DONG
1
;
Wan-Jun BAI
;
Hong-Tao LIU
;
Hao-Jing SONG
;
Zhi-Hong QIU
;
Jing AN
;
Shu-Hui ZHANG
Author Information
1. 河北省人民医院药学部
- Keywords:
digoxin;
enzyme multiplied immunoassay technique;
LC-MS/MS;
therapy drug monitor;
difference
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(7):640-642,651
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare liquid chromatography -tandem mass spectrometry ( LC-MS/MS ) and enzyme multiplied immunoassay technique ( EMIT) for the determination of digoxin in human plasma, and to evaluate the differences between two methods.Methods Method validation of LC -MS/MS was performed.A total of 75 samples from patients treated with digoxin were collected in therapeutic drug monitoring room.The results obtained by LC -MS/MS and EMIT were analyzed using paired t test.Results LC-MS/MS validation was consistent with the requirements of methodology.The results of 75 samples determined by two methods showed there was no statistical difference ( P>0.05 ) in low -concentration region.However, EMIT data in middle and high concentration region was significantly higher than that obtained by LC-MS/MS ( P <0.001 ).The mean differences between the two methods were (0.57 ±0.72) ng· mL-1 in middle-concentration region and (2.48 ±2.15 ) ng · mL-1 in high -concentration region, outside the clinically acceptable range.Conclusion Compared with the EMIT, LC-MS/MS is more suitable for clinical determination of the plasma concentrations of digoxin because of less interference of plasma immuno-reactive substances and more accurate quantitation.
