Inhibition effect of curcumin on the proliferation of ovarian carcinoma SKOV-3 cells
10.13699/j.cnki.1001-6821.2016.07.019
- VernacularTitle:姜黄素对卵巢癌细胞 SKOV－3增值抑制作用
- Author:
Gui-Xia SUN
1
;
Gui-Xia ZHANG
;
Chao GAO
;
Juan XIE
Author Information
1. 河南大学淮河医院 妇产科
- Keywords:
ovariancancer SKOV 3 cell;
curcumin;
cisplatine;
cell proliferation
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(7):637-639
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of curcumin on the proliferation of ovarian carcinoma SKOV-3 cells.Methods The model group ( including regular SKOV -3 cells only ) , blank group ( adding amount of nutrient solution) and experimental group were set in experiment.And the experimental group were divided into 7 groups ( the final concentrations of curcumin were 5,10,20,40,80 μmol· L-1 , there are C1 -C5 groups; cisplatin group ( 10 μmol · L-1 ) , 20 μmol · L-1 curcumin+10μmol· L-1 cisplatin group ( combination group) and each were built 6 complex holes.The inhibition effect of curcumin on the pro-liferation of SKOV-3 cells was determined with methythiazolyldiphenyl-tetrazoliumbromide( MTT).The apoptosis rate was determined with PI.The effect of the cell cycle was determined with propidium iodide ( PI ) staining.The the expression of cells in the Bcl -2 protein was deter-mined with ELLISA.Results The curcumin can inhibit the growth of SKOV3, which showed dose -dependent and time -dependent.The apoptosis rate in the model group was 0.15%, the C1 -C5 groups in experimental group were 3.86%, 26.98%, 33.76%, 47.75%, 68.86%,respectively;the cisplatin group was 53.91%, the combinationgroup was 77.96%.The number of the SKOV-3 cells in G0-G1 stage is increasing, in G2-M stage an S stage are in decline with the increasing concentration of curcumin.And the apoptosis rate is increasing ( P<0.05 ).And the curcumin can inhibit the expression of the SKOV -3 Bcl -2 protein, which showed the dose -dependent.Conclusion The curcumin can inhibit the proliferation of SKOV-3, which is co-effected by the induction of apopto-sis and the promotion of the cell cycle arrest.
