Effects of geraniin on the proliferation and osteogenic differentiation of murine bone marrow mesenchymal stem cells
10.13699/j.cnki.1001-6821.2016.07.018
- VernacularTitle:老鹳草素对小鼠骨髓基质干细胞增殖及成骨分化的影响
- Author:
Zhe ZHANG
1
;
Xiu-Juan XU
;
Xin LIU
;
Yan-Fen ZHANG
;
Si-Wen TANG
;
Hai-Rong WANG
Author Information
1. 河北省人民医院 省老年医学重点实验室
- Keywords:
geraniin;
bone marrow mesenchymal stem cell;
proliferation;
osteogenic differentiation
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(7):633-636
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of geraniin ( Ge) on the pro-liferation and osteogenic differentiation of murine bone marrow mesenchy-mal stem cells ( BMSCs).Methods BMSCs were isolated and cultured from mouse long bones.Four groups were designed, blank group (DMEM/F12 complete medium), Ge low,middle,high -doses groups (1 ×10 -9 ,1 ×10 -8 ,1 ×10 -7 mol· L-1 Ge+DMEM/F12 complete me-dium).Cell counting Kit-8 ( CCK-8) was used to detect the prolifera-tion of BMSCs.Flow cytometry was used to detect the cell cycle and apoptosis.Alkaline phosphatase ( ALP) detection kits were used for the detection of ALP activity.Alizarin Red-S method was used to study the mineralization of BMSCs.Results After 1, 3, 5 and 7 days of osteogenic induction, Ge dose -dependently promoted the proliferation and inhi-bited the cell apoptosis rate of BMSCs.Compared to the blank group (0.29 ±0.03), the A450value of BMSCs treated with three-dose Ge for 3 days were 0.41 ±0.06, 0.37 ±0.04 and 0.33 ±0.05, the difference was statistically significant ( P <0.05 ).Compared to the blank group (4.22 ±0.58)%, the cell apoptosis rate of BMSCs treated with three-dose Ge for 3 days were (2.53 ±0.40)%, (3.02 ±0.46)% and (3.71 ±0.54)%, the difference was statistically significant (P<0.05).Meanwhile S phase of cell cycle and the ALP activity increased, G0/G1 phase was down and the cell apoptosis decreased remarkably, the difference was statistically significant (P<0.05).After 21 day of osteogenic induction, Ge dose -dependently promoted the quantification of numbers of calcium nodules compared with blank group, the difference was statistically significant ( P<0.05 ).Conclusion Geraniin could promote proliferation via affecting cell cycle distribution and inhibiting BMSCs apoptosis and it could induce the osteogenic differentiation of BMSCs.
