Effects of Cinnamic aldehyde on the leukemia cell line K562 using Caco -2 cells in vitro absorption model
10.13699/j.cnki.1001-6821.2016.07.016
- VernacularTitle:桂皮醛通过 Caco－2细胞体外吸收模型对白血病K562细胞株的作用
- Author:
Xin GUAN
1
;
Tong-Hua YANG
;
Mian-Cheng SU
;
Ren-Bin ZHAO
;
Qiang PEI
;
Hong-Mei OUYANG
Author Information
1. 云南省第一人民医院血液科
- Keywords:
Cinnamic aldehyde;
K562 cell;
Caco-2 cell model;
surface antigen
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(7):625-628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study on the effects of Cinnamic aldehyde on leukemia cell line K562 by Caco -2 cells in vitro absorption model.Methods The effective components of cinnamon(0,50,100,200,400, 600,800,1000 μg· mL-1 ) were determined by Caco-2 cell model of Transwell, and the concentration was determined by HPLC.No cytotoxic concentration range of Cinnamic aldehyde acting on K562 cells for 72 h is detected by MTT assay.After 72 h incubation of Cinnamic aldehyde standard(50,75 μg· mL-1 ) and leukemia K562 cells, the cells surface antigens including CD235a, CD36, CD41, CD61, CD13, CD33 and CD14 were determined by Flow cytometry.Results The active ingredi-ent of cinnamon is extracted by transwell transport pool of Caco-2 cell model and no cytotoxic concentration is 200 μg · mL-1.The cinnamicaldehyde is the component which goes through the model by HPLC.The 24 h inhibition rates ( IRs ) of Cinnamic aldehyde on K562 cells are (25.29 ±0.97)%and (36.60 ±0.18)%at the concentrations of 50 and 75 μg· mL-1 , respectively;IRs for 48 h are ( 48.23 ±0.63 )% and ( 57.15 ±0.58 )%; IRs for 72 h are ( 58.23 ±0.63 )% and (57.15 ±0.58)%.Compared with the control group, the inhibitory activity is obvious(P<0.05).After incubation 72 h, the expressions of myeloid differentiation phenotypes including CD13, CD33, CD36 on K562 cells are (0.33 ±0.21)%, ( 32.89 ±0.19 )%, ( 7.73 ±0.57 )% and ( 0.72 ±0.43 )%, ( 38.80 ±0.03 )%, (10.90 ±0.82)%at the concentrations of 50 and 75 μg· mL-1 , respectively.Compared with the control group, the inhibition increased ( P <0.05 ).The phenotypic expressions of erythroid differentiation are ( 52.38 ±0.65 )%, (57.48 ±0.70)%.Compared with the control group, the inhibition increased( P<0.05).Megakaryocyte differentia-ted phenotype CD41, CD61 expression has no significant change ( P >0.05 ).Conclusion The Cinnamic aldehyde can go through the Caco-2 in vitro absorption model and enables the K562 cells to differentiate into myeloid and erythroid.
