Inhibition function of low molecular weight polysaccharide from Agaricus blazei on the apoptosis of hippocampal neurons in rats with oxygen stress
10.13699/j.cnki.1001-6821.2016.07.015
- VernacularTitle:低分子量姬松茸多糖抑制氧应激大鼠海马神经元的凋亡作用
- Author:
Li FAN
1
;
Shi-Ling LI
;
Chao YI
;
Li ZHOU
;
Yu ZOU
;
Gang LI
;
Li-Ling YUE
Author Information
1. 齐齐哈尔医学院 医药科学研究院
- Keywords:
hippocampal neurons;
oxidative stress;
low molecular weight polysaccharide from Agaricus blazei;
cell apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(7):622-624
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of low molecular weight polysaccharide from Agaricus blazei ( LMPAB ) on the expression of Bcl-2 and Bax in rat primary hippocampal neurons ( PHN) by oxidative stress.Methods PHN were clipped from 24 h -old rat and cultured primarily.Different groups were set, including the control group, model group and test groups (5,10 and 20 mg· L-1 ).At 24 h after LMPAB pretreatment, oxidative damage of cells were induced with 500μmol· L-1 H2 O2 for 2 h in model and test group.The PHN apoptosis and cell activity were measured with flow cytometry and MTT method.The expression of Bcl-2 and Bax protein were detected by immunocyto-chemical technique.Results Hippocampus neuron apoptosis rate in high dose test group (10.08 ±0.83) was significantly lower than that of model group ( 29.01 ±0.67 , P <0.01 ).Cell viability in high dose group ( 0.38 ±0.05 ) was significantly higher than that of model group (0.20 ±0.07, P <0.01 ) .Compared with model group, Bcl -2 expression significantly increased ( 42.73 ±3.65 vs 29.43 ±2.26 ).However, Bax expression significantly reduced in high dose group (22.47 ±2.82 vs 47.58 ±4.79)(P <0.01), Bcl -2/Bax increased.Conclusion LMPAB attenuated the hippcampal neurons oxidative damage induced by H2 O2.The mechanism may be related to its inhibition on the apoptosis of hippocampal neurons.
