Salvia miltiorrhizaligustrazine injection protects rat cerebral basal smooth muscle cells at low carbohydrates,hypoxia and reperfusion injury
10.13699/j.cnki.1001-6821.2016.07.014
- VernacularTitle:丹参川芎嗪注射液对缺氧／复氧损伤脑基底平滑肌细胞的保护作用
- Author:
Jian-Mei PEI
1
;
Yu HE
;
Bang-Hao ZHU
Author Information
1. 沈阳药科大学 生命科学与生物制药学院
- Keywords:
salvia miltiorrhizaligustrazine injection;
hypoxia;
cerebral basal smooth muscle cell;
protective effect
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(7):618-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore salvia miltiorrhizaligustrazine injection on cerebral basal smooth muscle cells under hypoxia ischemia and com-pare the efficacy of salvia miltiorrhiza, ligustrazine and salvia miltiorrhi-zaligustrazine injection.Methods Select cerebral basal smooth muscle cells to study.Use of sodium dithionite ( Na2 S2 O4 ) were incubated with the cells caused by oxygen and glucose deprivation injury.The experi-ment will be divided into control group ( fresh medium) , model group ( a lack of oxygen liquid) , salvia miltiorrhizaligustrazine low, middle, high-dose groups (0.01, 0.1, 1 μmol· L-1, 6 h), salvia miltiorrhiza group (0.1 μmol· L-1, 6 h), ligustrazine group(0.1 μmol· L-1, 6 h), salvia miltiorrhiza plus ligustrazine group ( 0.1 μmol · L-1 , 6 h ).Observe the general morphology of cerebral basal smooth muscle cells and morphological changes of apoptosis related, detection of cerebral basal smooth muscle cells survival, cerebral basal smooth muscle cells activity of extracellular lactate dehydrogenase ( LDH) and the activity of Bcl-2/Bax expression. Results Salvia miltiorrhizaligustrazine injection reduce hypoxia-reperfusion injury of cerebral basal smooth muscle cells in the general form.Compared with model group, it improve the survival rate of cerebral basal smooth muscle cells ( P<0.05 or 0.01 ) , increase the ratio of Bcl-2/Bax ( P<0.05 ) , and decrease the oxygen damage caused by extracellular LDH increased(P<0.05).Conclusion Salvia miltiorrhizaligustrazine in-jection has protective effect on the injury of cerebral basal smooth muscle cells induced by ischemia and hypoxia.Its protective effect superior to that with salvia miltiorrhiza andligustrazine.
