Effects of fructus corni officinalis fruit core extract on immune cytokine of adjuvant arthritis rats
10.13699/j.cnki.1001-6821.2016.06.023
- VernacularTitle:山茱萸果核提取物对佐剂性关节炎大鼠免疫调节因子的影响
- Author:
Jing-Jiao QI
1
;
Ping ZHANG
;
Jin-Wu GUO
;
Xi-Ming LU
Author Information
1. 河南科技大学 医学院 免疫教研室
- Keywords:
fructus corni officinalis fruit core extract;
adjuvant arthritis;
interleukin-17;
interleukin-23
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(6):549-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of fructus corni officinalis fruit core extract ( FCOFCE ) on immune cytokine of adjuvant arthritis rats, and to study the underlying anti -inflammatory mechanisms.Methods Adjuvant arthritis rat model was established with forty Wistar rats, after that, they were randomly divided into model group, triptery-gium wilfordii polyglycoside ( TWP) group, low-dose, medium-dose and high-dose of FCOFCE group, 8 rats in each group.Eight healthy Wistar rats was used as the normal control group.The normal group and model group were given the injection of 0.9%NaCl.TWP group was gi-ven the 0.01 mg· kg-1 TWP.Low-dose, medium-dose and high-dose of FCOFCE group were respectively given 200 , 400 and 600 mg· kg-1 FCOFCE.Six groups were given the corresponding dose by in-tragastric administration once a day, for 4 weeks.The swollen volume of rats’ rear claw were measured by plantar circumference.The interleukin ( IL)-23 mRNA and IL -17 mRNA expression in the blood lympho-cytes of adjuvant arthritis rats were detected by reversing transcription-polymerase chain reaction.Serum IL-23 and IL-17 were measured in all the sera with sandwich ELISA method.Results Compared with modelgroup, medium-dose and high-dose of FCOFCE group had remarkable decreasions in the terms of swollen volume of the rats’ rear claw, serum IL -17 and IL-23 contents and IL -17 mRNA and IL -23 mRNA expression levels ( P<0.05).No obvious differences were found between low-dose FCOFCE group and model group in the aforemen-tioned indexes ( P>0.05 ).Conclusion FCOFCE intervention can reduce the inflammatory reaction of adjuvant ar-thritis rats, the possible mechanism may be related to its down regulation of IL-17 and IL-23 levels.
