Effect of B-cell lymphoma/ieukemia-2 and hairy enhancer of split homologues 1 in rat cerebral ischemic preconditioning mediated neuroprotective
10.13699/j.cnki.1001-6821.2016.06.022
- VernacularTitle:B 细胞淋巴瘤－2原癌基因和发状分裂相关增强子－1在脑缺血预处理中介导神经保护的效应
- Author:
Li TIAN
1
;
Yu-An ZOU
;
Cai-Xia WU
;
Qian XUE
Author Information
1. 河北北方学院 研究生院
- Keywords:
ischemic preconditioning;
cerebral ischemic;
B-cell lymphoma /ieukemia-2;
hairy enhancer of split homologues 1;
neural protection
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(6):546-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mediated neuroprotective effect of B-cell lymphoma/Ieukemia -2 ( Bcl -2 ) and hairy enhancer of split homologues 1 ( Hes -1 ) after cerebral ischemic preconditioning.Methods The model A of cerebral ischemia was set up by the way of middle cerebral artery occlusion.The right internal carotid artery of rat was blocked transiently 10 minutes at a time, then after 3 days the model B was established by the way of model A.The sham operation group was only isolated carotid artery.The time 2, 3, 7, 14 days after the proce-dure , the neurobehavioral function in rat was evaluated by the way of Zea-Longa.The volume of cerebral infarction was measured by 2 , 3 , 5-triphenyltetrazolium chloride ( TTC) stain.The mRNA expression of Bcl-2 and Hes1 were assessed by real-time fluorescence quantitative polymerase chain reaction ( qRT -PCR ) .Results Compared with model A group, the neurologic impairment scores and cerebral infarction volume[ A:( 40.00 ±4.47 )%, B:( 19.00 ±2.75 )%, respectively ] of rats in model B group had obvious discrepancy( P<0.05).Hes1 mRNA expression in model A and model B were higher than sham operation group, model B were higher than model A, the difference had significantly (P<0.05).Bcl-2 mRNA expression, model B were significantly higher than sham group and model A at 2 days, the difference had significantly( P<0.05).Conclusion The Bcl-2 and Hes1 mRNA ex-pression increased after cerebral ischemia precondition, may be associated with nerve protective effect.
