Uncertainty evaluation on the determination of cantharidic acid in rat brain microdialysate by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2016.05.023
- VernacularTitle:HPLC－MS／MS法测定大鼠脑微透析液中斑蝥酸浓度的不确定度评定
- Author:
Jia-Shan ZHANG
1
;
Kun ZHAO
;
Xin-Gang LI
;
Sheng-Hui MEI
;
Zhi-Gang ZHAO
Author Information
1. 首都医科大学 附属北京天坛医院 药学部
- Keywords:
HPLC -MS/MS;
cantharidic acid;
microdialysis;
uncertainty
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(5):455-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the uncertainty on the determination of cantharidic acid ( CTR) in rat brain microdialysate by HPLC-MS/MS.Methods The uncertainty sources in the determination of CTR were analyzed and the uncertainty were evaluated and combined.Results The expanded uncertainty of CTR at low ( 0.19 ng· mL -1 ) and high ( 35.90 ng · mL -1 ) concentrations were 0.02 ng · mL -1 and 2.08 ng · mL -1 , respectively ( P =95%, k =2 ) .Conclusion The uncertainty on the determination of CTR in rat brain microdialysate by HPLC -MS/MS was mainly caused by matrix effect, calibration curve fitting, repeatability and sample preparation for low concentrations, and matrix effect, sample preparation and repeatability for high concentrations.
