Effect of cerebral ischemic preconditioning on content of amino acid and tumor necrosis factor-αin rats
10.13699/j.cnki.1001-6821.2016.05.013
- VernacularTitle:脑缺血预处理对大鼠脑组织中氨基酸及肿瘤坏死因子－α含量的影响
- Author:
Xiao-Qin WANG
1
;
Yu-An ZOU
;
Chun-Yan GUO
;
XUE-Qian
;
TIAN-Lin
;
Meng-Lin WEI
Author Information
1. 河北北方学院 研究生部
- Keywords:
cerebral ischemic preconditioning;
brain ischemic tolerance;
amino acid;
tumor necrosis factor -α
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(5):424-426
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the content change of glutamic acid ( Glu) , aspartic acid ( Asp) , glycine acid ( Gly) ,γ-aminobutyric acid ( GABA) , tumor necrosis factor -α( TNF-α) following a cerebral is-chemic preconditioning ( CIP) and its function.Methods A total of 90 male Sprague-Dawley rats were randomly divided into 3 groups:normal group ( n=30 ) , sham group ( n=30 ) and experiment group ( n=30 ).The three groups were divided into hour -1, hour -3, hour -6, hour-12 , hour -24 subgroups ( 6 in each subgroup ).The content of brain Glu, Asp, GABA, Gly were detected by HPLC.And TNF-αwas detected spectrophotography in rats with the ischemic preconditioning and sham operation at different times (1, 3, 6, 12, 24 h).Results Com-pared with those in the corresponding sham subgroups , the content of Glu, Asp, GABA, Gly began to significant increase at 1 h (P<0.05 or P<0.01 ) , peaked at 1 h or 3 h and decreased to basal at 3 h or 6 h After CIP.the content of TNF-αbegan to increase at 1 h ( P<0.05 ) , peaked at 6 h and not decreased to basal at 24 h After CIP.Conclusion A moderate increase in Glu, Asp, GABA, Gly, TNF-αafter CIP might play a important role in the induction of the early brain ischemic tolerance mechanism.
